FFPE Cell Pellet Scrolls Now Available from the NIGMS HGCR


Formalin-Fixed Paraffin-Embedded (FFPE) scrolls are now available for the cell line GM12878 via Coriell Institute’s online catalog. This product, Repository ID: FF12878, is available as a set of FFPE scrolls of a homogenous cell pellet of GM12878.

These FFPE scrolls from this well-characterized sample can be used to extract DNA and can be used in whole-genome sequencing and other genomic applications for pipeline validation and assay development. The parental cell line, GM12878, is a clinically relevant reference sample, validated across many NGS platforms for genomic testing.

FFPE cell pellet scrolls have particularly useful research applications alongside clinical FFPE tissue biopsies. This product can be used as an FFPE control sample, processed in parallel with a clinical FFPE tissue sample for genetic and genomic analyses. The process of fixing and embedding the reference cell line pellet mimics the preparation steps taken with tissue biopsies, thus subjecting the control sample to comparable physical and chemical stresses. These techniques, which are essential in clinical research, tend to compromise the final quality of extracted DNA, thus leading to a significant baseline performance difference as compared to a non-processed biospecimen. The use of an FFPE control sample therefore reduces technical variability between samples, improving the accuracy and reproducibility of clinical genomic tests.

See more detailed technical information about this sample below.

Product specifications:

Product: 3-4x FFPE scrolls shipped in a microcentrifuge tube
Sample type:
Formalin fixed, paraffin embedded cell pellet scrolls
Fixation method: 10% Neutral Buffered Formalin
Scroll size: 15μm length per scroll (3 scrolls included per sample) or 10μm length per scroll (4 scrolls included per sample)
Approximate cell density: ~3.5 x 10^5 cells per 15μm scroll or ~2.5x10^5 cells per 10μm scroll (obtained from an original FFPE block containing 3 x 10^8 cells per block)
Expected DNA yield: ≥300ng per 15μm scroll or ≥200ng per 10μm scroll using Promega Maxwell LEV Plus Extraction Kit
Sample storage: 4°C
DNA quality control: Extracted DNA measured for 260/280 & 260/230 ratios, Concentration, DNA Integrity Number

Other News