Repository NIGMS Human Genetic Cell Repository
Subcollection Hybrids
Cell Type Somatic cell hybrid
Transformant Untransformed
ISCN Human/hamster somatic cell hybrid retaining a human del(5)(p15.1) in 100% of cells
Remarks Line JH290; produced by the fusion of human lymphoblasts from a patient with Cri-du-Chat syndrome with Chinese hamster line UCW56 cells; grown in culture medium with 0.2mM proline at 39C; this hybrid retains a human del(5)(p15.1) in 100% of cells
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 
Remark Line JH290; produced by the fusion of human lymphoblasts from a patient with Cri-du-Chat syndrome with Chinese hamster line UCW56 cells; grown in culture medium with 0.2mM proline at 39C; this hybrid retains a human del(5)(p15.1) in 100% of cells
Overhauser J, Huang X, Gersh M, Wilson W, McMahon J, Bengtsson U, Rojas K, Meyer M, Wasmuth JJ, Molecular and phenotypic mapping of the short arm of chromosome 5: sublocalization of the critical region for the cri-du-chat syndrome. Hum Mol Genet3:247-52 1994
PubMed ID: 8004090
No data is available
Split Ratio 1:8
Temperature 39 C
Percent CO2 8%
Medium Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement Proline 0.2 mM