Repository NIGMS Human Genetic Cell Repository
Subcollection Heritable Diseases
Class Other Disorders of Known Biochemistry
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks Line RPMI8226; ATCC CCL155; IgG Lambda type light chain secreting; G6PD = A; negative for reverse transcriptase; modal no. of 65 chromosomes; range = 40 to 78; low TdT activity; negative for EBV and EBNA
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis
 
Remark Line RPMI8226; ATCC CCL155; IgG Lambda type light chain secreting; G6PD = A; negative for reverse transcriptase; modal no. of 65 chromosomes; range = 40 to 78; low TdT activity; negative for EBV and EBNA
Sanford JA, Stubblefield E, General protocol for microcell-mediated chromosome transfer. Somat Cell Mol Genet13:279-84 1987
PubMed ID: 3299749
 
Klobeck HG, Zimmer FJ, Combriato G, Zachau HG, Linking of the human immunoglobulin VK and JKCK regions by chromosomal walking. Nucleic Acids Res15:9655-65 1987
PubMed ID: 3122178
 
Mocikat R, Falkner FG, Mertz R, Zachau HG, Upstream regulatory sequences of immunoglobulin genes are recognized by nuclear proteins which also bind to other gene regions. Nucleic Acids Res14:8829-44 1986
PubMed ID: 3786139
 
Klobeck HG, Zachau HG, The human CK gene segment and the kappa deleting element are closely linked. Nucleic Acids Res14:4591-603 1986
PubMed ID: 3086844
 
Sutherland BM, Bennett PV, Freeman AG, Moore SP, Strickland PT, Transformation of human cells by DNAs ineffective in transformation of NIH 3T3 cells. Proc Natl Acad Sci U S A82:2399-403 1985
PubMed ID: 3857589
 
Goldstein M, Hoxie J, Zembryki D, Matthews D, Levinson AI, Phenotypic and functional analysis of B cell lines from patients with multiple myeloma. Blood66:444-6 1985
PubMed ID: 3893568
 
Lane MA, Sainten A, Cooper GM, Stage-specific transforming genes of human and mouse B- and T- lymphocyte neoplasms. Cell28:873-80 1982
PubMed ID: 6284377
 
Matsuoka Y, Moore GE, Yagi Y, Pressman D, Production of free light chains of immunoglobulin by a hematopoietic cell line derived from a patient with multiple myeloma. Proc Soc Exp Biol Med125:1246-50 1967
PubMed ID: 6042436
No data is available
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium