GM08145
LCL from B-Lymphocyte
Description:
CHROMOSOME DELETION
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Chromosome Abnormalities |
Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Family Member
|
3
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Relation to Proband
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mother
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Confirmation
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Clinical summary/Case history
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ISCN
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47,XX,del(17)(pter>p11.2::p10>qter),+der(17)(:p11.2>q10:)[39]/ 46,XX,del(17)(pter>p11.2::p10>qter)[11].ish del(17)(D17Z1+),der(17)(D17Z1+)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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Cytogenetics |
Chromosome 17: ANEUPLOID Aneuploid Segment (-)17p11>17cen |
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Chromosome 17: DELETION Aneuploid Segment (-)17p11>17cen |
Remarks |
Clinically normal; see GM08148 Fibroblast; 88% of peripheral blood lymphocytes had a 17p- marker chromosome; 60% of lymphoblasts have the del(17)(:p11.2> cen:) |
Wevrick R, Earnshaw WC, Howard-Peebles PN, Willard HF, Partial deletion of alpha satellite DNA associated with reduced amounts of the centromere protein CENP-B in a mitotically stable human chromosome rearrangement. Mol Cell Biol10:6374-80 1990 |
PubMed ID: 2247061 |
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Howard-Peebles, A stable supernumary chromosome derived from a deleted segment of 17p. Am J Hum Genet37:A97 (1985):6374-80 1985 |
PubMed ID: 2247061 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not Inactivated |
Substrate |
None specified |
Subcultivation Method |
dilution - add fresh medium |
Supplement |
- |
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