CRISPR Gene Engineering

The Coriell CRISPR Gene Engineering Core provides CRISPR gene-editing services to the research community. CRISPR technology is a simple yet powerful tool for editing genomes that allows researchers to easily alter DNA sequences and modify gene function. The ability to engineer biological systems and organisms holds enormous potential for applications across basic science, medicine, and biotechnology.*

The Coriell CRISPR Gene Engineering Core has tremendous experience with creating gene knock-outs and knock-ins. We used the CRISPR/Cas9 system, which can be delivered into cells in many forms — including plasmids, lentiviruses, adeno-associated viruses, and ribonucleoprotein (RNP) complexes. These systems for delivering CRISPR/Cas9 have been widely adopted to undertake both gene knockout and knock-in experiments in stable cell lines. We also have great experience in CRISPR-based screening with genome-wide pooled CRISPR guide RNA libraries — or sub-pool gRNA libraries — which are ideal for high-throughput screening of important molecular targets. These libraries leverage the efficiency and specificity of the CRISPR technology to either knock out gene expression or transcriptionally activate genes in the genome.

The Coriell CRISPR Gene Engineering Core offers a gRNA Design and Vector Construction Service, CRISPR/Cas9 Cell Line Engineering, and a CRISPR-based screening service with libraries for genome-wide knock-outs of human and mouse genes.

Contacts:

Dr. Jian Huang, 856-668-2075 Email

Dr. Zhaorui Lian, 856-757-9706 Email 

Huan Li, 856-757-9706 Email

*From "Beyond CRISPR: What’s current and upcoming in genome editing" on Science 2019