AG22160
Fibroblast from Skin, Toe
Description:
NON WOUND HEALING PHENOTYPE
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Non-Healing Wound Collection |
Biopsy Source
|
Toe
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Toe
|
Race
|
White
|
Family Member
|
1
|
Family History
|
N
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,Y,t(X;9)(q26;q32)[5]/46,XY[45]
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Senescence |
15 |
PDL at Freeze |
3.97 |
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
|
Remarks |
Culture established from explants of 1cm of skin punched out of an ulcer on the plantar surface of the right great toe; hypertension; myocardial infarction eleven years ago treated with two stents implanted during angioplasty; diabetic; melanoma removed from scalp with skin flap; ulcer had no pus, erythema or tenderness; subject denies trauma or pain and can ambulate; xray shows diffuse osteopenia in right foot with deformity with destruction of the first distal phalanx |
Brem H, Golinko MS, Stojadinovic O, Kodra A, Diegelmann RF, Vukelic S, Entero H, Coppock DL, Tomic-Canic M, Primary cultured fibroblasts derived from patients with chronic wounds: a methodology to produce human cell lines and test putative growth factor therapy such as GMCSF Journal of translational medicine6:75 2008 |
PubMed ID: 19046453 |
PDL at Senescence |
15 |
Cumulative PDL at Freeze |
3.97 |
Passage Frozen |
2 |
Split Ratio |
1:4 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Supplement |
- |
|
|