AG22586
Fibroblast from Skin, Foreskin
Description:
APPARENTLY HEALTHY INDIVIDUAL
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Apparently Healthy Collection |
Biopsy Source
|
Foreskin
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Foreskin
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XY
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Senescence |
25 |
PDL at Freeze |
2.41 |
Passage Frozen |
1 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
|
Remarks |
Foreskin fibroblast cell culture |
Patgiri A, Skinner OS, Miyazaki Y, Schleifer G, Marutani E, Shah H, Sharma R, Goodman RP, To TL, Robert Bao X, Ichinose F, Zapol WM, Mootha VK, An engineered enzyme that targets circulating lactate to alleviate intracellular NADH:NAD Nature biotechnology: 2019 |
PubMed ID: 31932725 |
PDL at Senescence |
25 |
Cumulative PDL at Freeze |
2.41 |
Passage Frozen |
1 |
Split Ratio |
1:20 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Supplement |
- |
|
|