The Allen Institute for Cell Science is dedicated to understanding and predicting behavior of cells during normal, regenerative, and pathological states by live imaging of fluorescently-tagged human induced pluripotent stem cells and generating tools and multi-scale visual models of cell organization to encourage experimental observation, theory and prediction. The Allen Cell Collection at the Coriell Institute supports this mission through world-wide distribution of this continually growing collection of high quality-certified fluorescently-tagged hiPSC lines to academic researchers and commercial entities.
The Allen Cell Collection is a bank of human induced pluripotent stem cells that are currently derived from the WTC parental line released by the Conklin Laboratory at the J. David Gladstone Institute (available through Coriell as GM25256, where you can also find the de-identified patient information associated with the line). Please refer to the Allen Institute for Cell Science publication for more details on how these cells lines were generated along with the extensive quality control data (Roberts et al., MBoC 2017).
To answer the most basic questions about human health and disease, we need to understand how the dynamic organization and activities of the cell drive its function. This set of powerful gene edited human
stem cells from the Allen Institute for Cell Science is the first publicly available collection of hiPSC lines with key structural proteins fluorescently tagged.
The Disease Collection Cell Catalog is a growing compilation of cell line collections that carry mutations in genes known to cause disease. These cell lines were created by introducing a point mutation in one of the fluorescently tagged WTC-11 clonal lines from the Allen Cell Collection. The Disease Collection presently encompasses disease models for cardiomyopathy, laminopathy, and skeletal myopathy, each paired with isogenic variants that can accurately replicate the diverse phenotypic outcomes resulting from the mutations. The number of isogenic variants differs between collections and cannot be purchased separately.
All gene editing and quality control were performed by the Allen Institute for Cell Science.
The Allen Cell Collection is now available to academic researchers and commercial entities! New lines continue to be released several times a year, so stay tuned for new cell lines.
Cell Catalog Update
Two new fluorescently tagged hiPSC lines are now available in the Allen Cell Collection. The new cell lines include a single and a dual tag localized at the mitochondrial. The single tag contains mTagRFP-T localized to the mitochondrial matrix via COXVIII. The dual tag includes mTagRFP-T-COXVIII, and TOMM20 is endogenously tagged with mEGFP.
Allen Cell Collection
Now has 60 high quality-certified hiPSC lines available to help your research program. The focus of the Collection is fluorescently-tagged hiPSC lines that target 44 key cellular structures/substructures. The Allen Cell Collection is now available to academic researchers and commercial entities! New lines continue to be released several times a year, so stay tuned for new cell lines.
Please pay special attention when ordering either of the endosome, Golgi or adherens junctions (E-cadherin and VE-cadherin) cell lines as the AICS number is now qualified with a three-digit clonal extension to help discriminate between the mono- and bi-allelic edits of the same structure.
Newest lines available in 2024:
• WTC Dual tagged TOMM20-mEGFP/mTagRFP-T-COXVIII
Coming soon:
• TBR2-mEGFP
Disease Collection:
The recent release includes 3 different disease models and 8 different collections with different point mutations with corresponding isogenic variants - a total of 36 cell lines. Listed below are the protein changes resulting from the point mutation.
• Cardiomyopathy (MYH7) – 6 new collections:
• Laminopathy (LMNA) – 1 new collection:
• Skeletal Myopathy (MYH3) – 1 new collection:
Quality Assurance
Each cell line has undergone a broad range of quality control experiments to ensure precise insertion of the fluorescent tag and localization of the tagged protein to the correct cellular compartment without disrupting the cells’ overall genomic integrity. More details about how these lines are generated and the Quality Control steps involved in their generation are available by clicking on the AICS link provided for each line in the “Order Page” for each cell line.
Organization and Contact Information
| Allen Institute | Coriell Institute |
| Irina Mueller, PhD | Adam Green |
| Director, Scientific Operations | Project Lead |
| Email: cells@alleninstitute.org | Email: agreen@coriell.org |
