Passage Frozen	37
Induced Pluripotent Stem Cell	The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.
IDENTIFICATION OF SPECIES OF ORIGIN	Species of Origin Confirmed by LINE assay

Remark

Induced pluripotent stem cell line derived from adult skin (leg) fibroblasts; subject clinically normal; wild-type; FISH test: 46, XY. This iPSC line (WTC-11) was submitted by Dr. Bruce R. Conklin (Gladstone Institute of Cardiovascular Disease, UCSF) and may serve as a control for diseased iPSCs GM25267 (LQT2) iPSC and GM25305 (LQT3); also available is a collection of fluorescently tagged hiPSC lines from the same subject, using CRISPR/Cas9 technology (for more information, see the Allen Cell Collection at Coriell: https://catalog.coriell.org/1/AllenCellCollection).

Gong S, Li Y, Su W, Ding Y, Lu J, Dong K, Hood S, Zhang W, Terstappen GC, Quantitative Algorithm-Based Paired Imaging Measurement for Antibody-Triggered Endocytosis in Cultured Cells SLAS discovery : advancing life sciences R & D:2472555218761355 2018

PubMed ID: 29505735

Hoang P, Wang J, Conklin BR, Healy KE, Ma Z, Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells Nature protocols13:723-737 2018

PubMed ID: 29543795

Libby AR, Joy DA, So PL, Mandegar MA, Muncie JM, Mendoza-Camacho FN, Weaver VM, Conklin BR, McDevitt TC, Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference Elife13:723-737 2018

PubMed ID: 30298816

Colasuonno F, Borghi R, Niceforo A, Muzzi M, Bertini E, Di Giulio A, Moreno S, Compagnucci C, Senescence-associated ultrastructural features of long-term cultures of induced pluripotent stem cells (iPSCs) Aging13:723-737 2017

PubMed ID: 29064821

Huebsch N, Loskill P, Mandegar MA, Marks NC, Sheehan AS, Ma Z, Mathur A, Nguyen TN, Yoo JC, Judge LM, Spencer CI, Chukka AC, Russell CR, So PL, Conklin BR, Healy KE, Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales Tissue engineering Part C, Methods21:467-79 2015

PubMed ID: 25333967

Kreitzer FR, Salomonis N, Sheehan A, Huang M, Park JS, Spindler MJ, Lizarraga P, Weiss WA, So PL, Conklin BR, A robust method to derive functional neural crest cells from human pluripotent stem cells American journal of stem cells2:119-31 2013

PubMed ID: 23862100

Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR, Isolation of single-base genome-edited human iPS cells without antibiotic selection Nature methods11:291-3 2013

PubMed ID: 24509632

No data is available

No data is available

Passage Frozen	37
Split Ratio	1:8
Temperature	37 C
Percent CO2	5%
Percent O2	AMBIENT
Medium	mTeSR1
Serum	none
Substrate	Matrigel