GM25256
iPSC from Fibroblast
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection
PIGI Consented Sample |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Tissue Type
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Episomal)
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Race
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Asian
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Hispanic Ethnicity
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Not Hispanic/Latino
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Ethnicity
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Japanese
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Country of Origin
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USA
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis before cell line submission to CCR
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ISCN
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46,XY[25].arr[hg19]7q11.22(69,305,809-70,157,870)x1
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
37 |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Remark |
Induced pluripotent stem cell line derived from adult skin (leg) fibroblasts; subject clinically normal; wild-type; FISH test: 46, XY. This iPSC line (WTC-11) was submitted by Dr. Bruce R. Conklin (Gladstone Institute of Cardiovascular Disease, UCSF) and may serve as a control for diseased iPSCs GM25267 (LQT2) iPSC and GM25305 (LQT3); also available is a collection of fluorescently tagged hiPSC lines from the same subject, using CRISPR/Cas9 technology (for more information, see the Allen Cell Collection at Coriell: https://catalog.coriell.org/1/AllenCellCollection). |
| Li L, Li Y, Sottas C, Culty M, Fan J, Hu Y, Cheung G, Chemes HE, Papadopoulos V, Directing differentiation of human induced pluripotent stem cells toward androgen-producing Leydig cells rather than adrenal cells Proceedings of the National Academy of Sciences of the United States of America: 2019 |
| PubMed ID: 31591190 |
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| Schneider O, Zeifang L, Fuchs S, Sailer C, Loskill P, User-Friendly and Parallelized Generation of Human Induced Pluripotent Stem Cell-Derived Microtissues in a Centrifugal Heart-on-a-Chip Tissue engineering Part A25:786-798 2019 |
| PubMed ID: 30968738 |
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| Madsen RR, Semple RK, Luminescent peptide tagging enables efficient screening for CRISPR-mediated knock-in in human induced pluripotent stem cells Wellcome open research4:37 2019 |
| PubMed ID: 31363496 |
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| Regier MC, Tokar JJ, Warrick JW, Pabon L, Berthier E, Beebe DJ, Stevens KR, User-defined morphogen patterning for directing human cell fate stratification Scientific reports9:6433 2019 |
| PubMed ID: 31015521 |
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| Libby ARG, Briers D, Haghighi I, Joy DA, Conklin BR, Belta C, McDevitt TC, Automated Design of Pluripotent Stem Cell Self-Organization Cell systems9:483-495.e10 2019 |
| PubMed ID: 31759947 |
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| Bertero A, Fields PA, Ramani V, Bonora G, Yardimci GG, Reinecke H, Pabon L, Noble WS, Shendure J, Murry CE, Dynamics of genome reorganization during human cardiogenesis reveal an RBM20-dependent splicing factory Nature communications10:1538 2018 |
| PubMed ID: 30948719 |
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| Miklas JW, Clark E, Levy S, Detraux D, Leonard A, Beussman K, Showalter MR, Smith AT, Hofsteen P, Yang X, Macadangdang J, Manninen T, Raftery D, Madan A, Suomalainen A, Kim DH, Murry CE, Fiehn O, Sniadecki NJ, Wang Y, Ruohola-Baker H, TFPa/HADHA is required for fatty acid beta-oxidation and cardiolipin re-modeling in human cardiomyocytes Nature communications10:4671 2018 |
| PubMed ID: 31604922 |
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| Gong S, Li Y, Su W, Ding Y, Lu J, Dong K, Hood S, Zhang W, Terstappen GC, Quantitative Algorithm-Based Paired Imaging Measurement for Antibody-Triggered Endocytosis in Cultured Cells SLAS discovery : advancing life sciences R & D10:2472555218761355 2018 |
| PubMed ID: 29505735 |
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| Hoang P, Wang J, Conklin BR, Healy KE, Ma Z, Generation of spatial-patterned early-developing cardiac organoids using human pluripotent stem cells Nature protocols13:723-737 2018 |
| PubMed ID: 29543795 |
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| Libby AR, Joy DA, So PL, Mandegar MA, Muncie JM, Mendoza-Camacho FN, Weaver VM, Conklin BR, McDevitt TC, Spatiotemporal mosaic self-patterning of pluripotent stem cells using CRISPR interference Elife13:723-737 2018 |
| PubMed ID: 30298816 |
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| Colasuonno F, Borghi R, Niceforo A, Muzzi M, Bertini E, Di Giulio A, Moreno S, Compagnucci C, Senescence-associated ultrastructural features of long-term cultures of induced pluripotent stem cells (iPSCs) Aging13:723-737 2017 |
| PubMed ID: 29064821 |
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| Barber K, Studer L, Fattahi F, Derivation of enteric neuron lineages from human pluripotent stem cells Nature protocols14:1261-1279 2016 |
| PubMed ID: 30911172 |
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| Huebsch N, Loskill P, Mandegar MA, Marks NC, Sheehan AS, Ma Z, Mathur A, Nguyen TN, Yoo JC, Judge LM, Spencer CI, Chukka AC, Russell CR, So PL, Conklin BR, Healy KE, Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales Tissue engineering Part C, Methods21:467-79 2015 |
| PubMed ID: 25333967 |
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| Kreitzer FR, Salomonis N, Sheehan A, Huang M, Park JS, Spindler MJ, Lizarraga P, Weiss WA, So PL, Conklin BR, A robust method to derive functional neural crest cells from human pluripotent stem cells American journal of stem cells2:119-31 2013 |
| PubMed ID: 23862100 |
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| Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR, Isolation of single-base genome-edited human iPS cells without antibiotic selection Nature methods11:291-3 2013 |
| PubMed ID: 24509632 |
| Passage Frozen |
37 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none |
| Substrate |
Matrigel |
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