Description:
DELTA-BETA THALASSEMIA
HEMOGLOBIN--BETA LOCUS; HBB
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Heritable Diseases |
| Class |
Mutations of the Hemoglobin Loci |
| Quantity |
25 µg |
| Quantitation Method |
Please see our FAQ |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
DNA from LCL
|
|
Race
|
White
|
|
Ethnicity
|
SICILIAN
|
|
Family Member
|
2
|
|
Relation to Proband
|
sister
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this cell culture gave a negative result with a primer for Yq11, DYS227. |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Sicilian; homozygous delta-beta thalassemia; the 3' breakpoint is located approximately 5 kb to the 3' side of the B-globin gene; the overall deletion comprises approximately 13 kb |
| Reyes AA, Carrera P, Cardillo E, Ugozzoli L, Lowery JD, Lin CI, Go M, Ferrari M, Wallace RB, Ligase chain reaction assay for human mutations: the Sickle Cell by LCR assay. Clin Chem43:40-4 1997 |
| PubMed ID: 8990220 |
| |
| Tuan D, Feingold E, Newman M, Weissman SM, Forget BG, Different 3' end points of deletions causing delta beta-thalassemia and hereditary persistence of fetal hemoglobin: implications for the control of gamma-globin gene expression in man. Proc Natl Acad Sci U S A80:6937-41 1983 |
| PubMed ID: 6196781 |
| |
| Mears JG, Ramirez F, Leibowitz D, Nakamura F, Bloom A, Konotey-Ahulu F, Bank A, Changes in restricted human cellular DNA fragments containing globin gene sequences in thalassemias and related disorders. Proc Natl Acad Sci U S A75:1222-6 1978 |
| PubMed ID: 274714 |
|
|