Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
PIGI Consented Sample
Quantity 10 µg
Quantitation Method Please see our FAQ
Biopsy Source Arm
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Race Caucasian
Country of Origin USA
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks Participant #1 (hu43860C) in the Personal Genome Project: http://www.personalgenomes.org Matching lymphoblast culture from this same subject is GM20431.
PDL at Freeze 5.68
Passage Frozen 6
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by LINE assay
 
Remark Participant #1 (hu43860C) in the Personal Genome Project: http://www.personalgenomes.org Matching lymphoblast culture from this same subject is GM20431.
Sauter EJ, Kutsche LK, Klapper SD, Busskamp V, Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders Methods in molecular biology (Clifton, NJ)1942:101-121 2019
PubMed ID: 30900179
 
Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19:101-121 2018
PubMed ID: 30567591
 
Drouin-Ouellet J, Pircs K, Barker RA, Jakobsson J, Parmar M, Direct Neuronal Reprogramming for Disease Modeling Studies Using Patient-Derived Neurons: What Have We Learned? Frontiers in neuroscience11:530 2017
PubMed ID: 29033781
 
Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016
PubMed ID: 27724973
 
Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005
PubMed ID: 16729065
No data is available
Passage Frozen 6
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Supplement Epidermal Growth Factor 0.1%
Supplement Non-Essential Amino Acids 1%