Repository NIGMS Human Genetic Cell Repository
Subcollection Apparently Healthy Collection
PIGI Consented Sample
Whole-Genome Sequence
Quantity 50 µg
Quantitation Method Please see our FAQ
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Race Caucasian
Country of Origin USA
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN 46,XY.arr[hg19]9p24.2p24.1(3,957,865-5,014,856)x1,9p24.1p23(5,039,328-10,280,656)x1
Species Homo sapiens
Common Name Human
Remarks Participant #1 (hu43860C) in the Personal Genome Project: http://www.personalgenomes.org Matching fibroblast culture from this same subject is GM23248. Whole genome sequence data is available on the PGP website.
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin confirmed by LINE assay
 
Demographic Data
Relation to Proband proband
Age at Sampling 51 YR
Gender Male
Racial Category Caucasian
Country USA
 
Data Elements
Clinical Element Type: Church PHGP
  (Baseline)
Vital Signs
Blood Type  O+
Height  6'4"
Weight  245
Blood Pressure - Systolic (mm Hg)  120
Blood Pressure - Diastolic (mm Hg)  80
Allergies
Substance  NONE
Medications, Vitamins, Supplements
Medication  LOVASTATIN, COQ (100MG) CALCIUM + VIT D, VIT C, OMEGA FATTY ACIDS
Medical History
Medical Condition  NARCOLEPSY, HYPERLIPIDEMIA, IRITIS, HYPERSOMNOLENCE, SQUAMOUS CELL CARCINOMA, CHEST PAIN
Race, Ethnicity, Ancestry
Race  WHITE
Country of Origin - Subject  USA
Country of Origin - Paternal Grandfather  USA
Country of Origin - Paternal Grandmother  USA
Country of Origin - Maternal Grandfather  USA
Country of Origin - Maternal Grandmother  USA
Traits
Eye Color  green
Dominant Hand  right
Near-sighted Yes   No 
Far-sighted Yes   No 
Wear Prescription Glasses Yes   No 
Remark Participant #1 (hu43860C) in the Personal Genome Project: http://www.personalgenomes.org Matching fibroblast culture from this same subject is GM23248. Whole genome sequence data is available on the PGP website.
Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016
PubMed ID: 27724973
 
Peters BA1, Kermani BG, Sparks AB, Alferov O, Hong P, Alexeev A, Jiang Y, Dahl F, Tang YT, Haas J, Robasky K, Zaranek AW, Lee JH, Ball MP, Peterson JE, Perazich H, Yeung G, Liu J, Chen L, Kennemer MI, Pothuraju K, Konvicka K, Tsoupko-Sitnikov M, Pant KP, Ebert JC, Nilsen GB, Baccash J, Halpern AL, Church GM, Drmanac R., Accurate whole-genome sequencing and haplotyping from 10 to 20 human cells. Nature487(7406):190-5 2012
PubMed ID: 22785314
 
Drmanac R1, Sparks AB, Callow MJ, Halpern AL, Burns NL, Kermani BG, Carnevali P, Nazarenko I, Nilsen GB, Yeung G, Dahl F, Fernandez A, Staker B, Pant KP, Baccash J, Borcherding AP, Brownley A, Cedeno R, Chen L, Chernikoff D, Cheung A, Chirita R, Curson B, Ebert JC, Hacker CR, Hartlage R, Hauser B, Huang S, Jiang Y, Karpinchyk V, Koenig M, Kong C, Landers T, Le C, Liu J, McBride CE, Morenzoni M, Morey RE, Mutch K, Perazich H, Perry K, Peters BA, Peterson J, Pethiyagoda CL, Pothuraju K, Richter C, Rosenbaum AM, Roy S, Shafto J, Sharanhovich U, Shannon KW, Sheppy CG, Sun M, Thakuria JV, Tran A, Vu D, Zaranek AW, Wu X, Drmanac S, Oliphant AR, Banyai WC, Martin B, Ballinger DG, Church GM, Reid CA., Human genome sequencing using unchained base reads on self-assembling DNA nanoarrays. Science327(5961):78-81 2010
PubMed ID: 19892942
 
Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:78-81 2005
PubMed ID: 16729065
View karyotype 
karyotype 
Split Ratio 1:4
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium