GM17552
LCL from B-Lymphocyte
Description:
IMMUNODEFICIENCY WITH HYPER-IGM, TYPE 2
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Disorders of Uncertain Biochemical Etiology |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line LAZ187; at age 7 patient had recurrent pyogenic infections; IgG<100mg/dl; IgM>50mg/dl (sometimes as high as 200); IgA<50mg/dl; the cells express surface IgM and IgD, but not IgG |
| Schwaber JF, Rosen FS, Isotypes of surface immunoglobulin on B lymphocytes from patients with immune deficiency. J Clin Immunol2(1):30-4 1982 |
| PubMed ID: 6980225 |
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| Schwaber JF, Lazarus H, Rosen FS, IgM-restricted production of immunoglobulin by lymphoid cell lines from patients with immunodeficiency with hyper IgM (dysgammaglobulinemia). Clin Immunol Immunopathol19(1):91-7 1981 |
| PubMed ID: 7011617 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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