GM14050
Somatic cell hybrid
Description:
CHROMOSOME 13 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/mouse hybrid retaining human der(X)t(X;13)(p22;q12),del(2p), 3, 14, 18, & 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phophorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xq26, HPRT, Xq13.3, PGK1, and Xq21.3, DXS3, and gave a negative result with a primer for Xp22.32, STS. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 13 |
PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 13q14.3, RB1, 13q22-q31, D13S317, and 13q34, F7.1, and gave a negative result with a primer for 13q11-q12, D13S232. |
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| Remarks |
Line CF25; produced by the fusion of human fibroblasts, 46,X,t(X;13)(p22;q12), with HPRT-deficient mouse A9 cells; grown in DMEM medium with HAT; retains the der(X)t(X;13) in 100% of cells & other human chromosomes |
| Warburton D, Yu MT, Tantravahi U, Lee C, Cayanis E, Russo J, Fischer SG, Regional localization of 32 NotI-HindIII fragments from a human chromosome 13 library by a somatic cell hybrid panel and in situ hybridization. Genomics16:355-60 1993 |
| PubMed ID: 8314575 |
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| Washington SS, Bowcock AM, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence SL, Cowell J, Ledbetter DH, White RL, Chakravarti A, A somatic cell hybrid map of human chromosome 13. Genomics18:486-95 1993 |
| PubMed ID: 8307557 |
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| Mohandas T, Sparkes RS, Shapiro LJ, Genetic evidence for the inactivation of a human autosomal locus attached to an inactive X chromosome. Am J Hum Genet34:811-7 1982 |
| PubMed ID: 7124733 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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