Repository NIGMS Human Genetic Cell Repository
Subcollection Heritable Diseases
Alternate IDs GM17346 [HLA HISTOCOMPATIBILITY TYPE: 142800 THROUGH 142860 & 142880]
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Race Black
Ethnicity AFRICAN
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks B cell line, no HLA: A, B, or C; no B2 Microglobulin; HLA: D:6; line DAUDI; culture is negative for T cell 65,000 MW glycoprotein; positive for sIg and Ia (B cell) antigen; weak response to anti-HLA antibody
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME PCR analysis of DNA from this cell culture gave a positive result with a primer for Yq11, DYS227.
HLA TYPING No HLA A, B, or C; No Beta 2 microglobulin; HLA D:6
Remark B cell line, no HLA: A, B, or C; no B2 Microglobulin; HLA: D:6; line DAUDI; culture is negative for T cell 65,000 MW glycoprotein; positive for sIg and Ia (B cell) antigen; weak response to anti-HLA antibody
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PubMed ID: 6197968
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PubMed ID: 6415171
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PubMed ID: 7017212
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PubMed ID: 6935474
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PubMed ID: 77067
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PubMed ID: 307019
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PubMed ID: 200169
Klein E, Klein G, Nadkarni JS, Nadkarni JJ, Wigzell H, Clifford P, Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res28:1300-10 1968
PubMed ID: 4174339
No data is available
Split Ratio 1:4
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium