Repository NIGMS Human Genetic Cell Repository
Subcollection Heritable Diseases
Alternate IDs GM17346 [HLA HISTOCOMPATIBILITY TYPE: 142800 THROUGH 142860 & 142880]
Biopsy Source Peripheral vein
Cell Type B-Lymphocyte
Tissue Type Blood
Transformant Epstein-Barr Virus
Race Black
Ethnicity AFRICAN
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks B cell line, no HLA: A, B, or C; no B2 Microglobulin; HLA: D:6; line DAUDI; culture is negative for T cell 65,000 MW glycoprotein; positive for sIg and Ia (B cell) antigen; weak response to anti-HLA antibody
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME PCR analysis of DNA from this cell culture gave a positive result with a primer for Yq11, DYS227.
 
HLA TYPING No HLA A, B, or C; No Beta 2 microglobulin; HLA D:6
 
Remark B cell line, no HLA: A, B, or C; no B2 Microglobulin; HLA: D:6; line DAUDI; culture is negative for T cell 65,000 MW glycoprotein; positive for sIg and Ia (B cell) antigen; weak response to anti-HLA antibody
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PubMed ID: 16251457
 
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PubMed ID: 12196404
 
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PubMed ID: 2435832
 
Engelhard VH, Benjamin C, Xenogeneic cytotoxic T-cell clones recognize alloantigenic determinants on HLA-A2. Immunogenetics18:461-473 1983
PubMed ID: 6196283
 
Ways JP, Parham P, The binding of monoclonal antibodies to cell-surface molecules. A quantitative analysis with immunoglobulin G against two alloantigenic determinants of the human transplantation antigen HLA-A2. Biochem J216:423-32 1983
PubMed ID: 6197968
 
Shipp MA, Schwartz BD, Kannapell CC, Griffith RC, Scott MG, Ahmed P, Davie JM, Nahm MH, A unique DR-related B cell differentiation antigen. J Immunol131:2458-67 1983
PubMed ID: 6415171
 
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PubMed ID: 7017212
 
Wright WC, Daniels WP, Fogh J, Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J Natl Cancer Inst66:239-47 1981
PubMed ID: 6935474
 
Arce-Gomez B, Jones EA, Barnstable CJ, Solomon E, Bodmer WF, The genetic control of HLA-A and B antigens in somatic cell hybrids: requirement for beta2 microglobulin. Tissue Antigens11:96-112 1978
PubMed ID: 77067
 
Carrel S, Gross N, Salvi S, Rabbit antiserum against Daudi-cell membrane fractions detects cell surface antigens present on subpopulations of human lymphocytes. J Immunol120:1587-93 1978
PubMed ID: 307019
 
Swallow DM, Gardiner SE, Harris H, Arthur E, Steel CM, Evans HJ, Lysosomal enzymes in human lymphoblastoid lines: unusual characteristics of RAJI and DAUDI. Ann Hum Genet41:9-16 1977
PubMed ID: 200169
 
Klein E, Klein G, Nadkarni JS, Nadkarni JJ, Wigzell H, Clifford P, Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res28:1300-10 1968
PubMed ID: 4174339
No data is available
Split Ratio 1:4
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not Inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium