GM29744
Fibroblast from Skin, Skin
Description:
FRONTOTEMPORAL DEGENERATION
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Human Variation |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Skin
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Race
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White
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Subject Type
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family with at least 3 members, including 1 proband, not a trio
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Family Type
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NUCLEAR FAMILIES - ONE AFFECTED
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Country of Origin
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USA
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Family Member
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2
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Family History
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Y
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Relation to Proband
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unaffected spouse
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Confirmation
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Molecular characterization after cell line submission to CCR
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5.63 |
| Passage Frozen |
6 |
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| Remarks |
Clinically unaffected spouse; No mutation in the MAPT gene confirmed with PCR; Affected spouse: GM29745; At risk daughter: GM29746; Clinically unaffected son: GM29747; Clinically unaffected daughter: GM29748. |
| Passage Frozen |
6 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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