Description:
NEUROBLASTOMA; NB NEUROBLASTOMA SUPPRESSOR, INCLUDED; NBS, INCLUDED
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
Chromosome Abnormalities |
| Class |
Disorders of the Nervous System |
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Cell Type
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Fibroblast
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Tissue Type
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Tumor
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Transformant
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Untransformed
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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47,X,-Y,+13,+19p+,9q-,11q-,19p+
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Stage IV; patient expired at age 16 months; at 15 months, developed 'raccoon eyes' and bone metastases; monolayer culture, which may pile up, grows slowly; originated from tumor tissue passaged in a nude mouse; 47,X,-Y, +13,+19p+,9q-,11q-,19p+; pass 1 at CCR |
| Colella R, Lu G, Glazewski L, Korant B, Matlapudi A, England MR, Craft C, Frantz CN, Mason RW, Induction of cell death in neuroblastoma by inhibition of cathepsins B and L Cancer letters294:195-203 2009 |
| PubMed ID: 20362389 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 Medium/RPMI 1640 Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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