GM08206
Fibroblast from Tumor, Hepatoblastoma
Description:
BECKWITH-WIEDEMANN SYNDROME; BWS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
dbGaP |
| Class |
Other Disorders of Known Biochemistry |
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Biopsy Source
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Hepatoblastoma
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Cell Type
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Fibroblast
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Tissue Type
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Tumor
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Tumor, Hepatoblastoma
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
11 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Culture established from a liver hepatoblastoma; macroglossia; hypoglycemia; bilateral enlarged kidneys; normal bone age; typical facies: large, thickened protruding tongue & prominent jaw; umbilical hernia; 46,XY |
| Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013 |
| PubMed ID: 23665875 |
| Passage Frozen |
11 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Williams Medium E |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Insulin, Transferrin, Selenium 100x |
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