GM05372
LCL from B-Lymphocyte
Description:
HUMAN ERYTHROLEUKEMIC CELL LINES
LEUKEMIA, CHRONIC MYELOID; CML
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Mutations of the Hemoglobin Loci |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line K562; established from a patient with chronic myelogenous leukemia by Lozzio and Lozzio; produces embryonic hemoglobin in response to hemin; modal number of chromosomes = 60; range is 55-62; structural aberrations for chromosomes #1, #3, #9, #13, and #14 were observed |
| Funk RS, Singh RK, Becker ML, Metabolomic Profiling to Identify Molecular Biomarkers of Cellular Response to Methotrexate In Vitro Clinical and translational science: 2019 |
| PubMed ID: 31651077 |
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| Gervais FG, Thornberry NA, Ruffolo SC, Nicholson DW, Roy S, Caspases cleave focal adhesion kinase during apoptosis to generate a FRNK-like polypeptide. J Biol Chem273:17102-8 1998 |
| PubMed ID: 9642276 |
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| ten Hoeve J, Morris C, Poustka A, Groffen J, Heisterkamp N, Isolation of NotI sites from chromosome 22q11. Genomics18:588-97 1993 |
| PubMed ID: 7905853 |
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| Mononen I, Heisterkamp N, Kaartinen V, Mononen T, Williams JC, Groffen J, Aspartylglycosaminuria in a non-Finnish patient caused by a donor splice mutation in the glycoasparaginase gene. J Biol Chem267:3196-9 1992 |
| PubMed ID: 1737774 |
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| Mononen I, Heisterkamp N, Kaartinen V, Williams JC, Yates JR 3d, Griffin PR, Hood LE, Groffen J, Aspartylglycosaminuria in the Finnish population: identification of two point mutations in the heavy chain of glycoasparaginase. Proc Natl Acad Sci U S A88:2941-5 1991 |
| PubMed ID: 2011603 |
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| Suedhoff T, Birckbichler PJ, Lee KN, Conway E, Patterson MK Jr, Differential expression of transglutaminase in human erythroleukemia cells in response to retinoic acid. Cancer Res50:7830-4 1990 |
| PubMed ID: 1979250 |
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| Hess J, Perez-Stable C, Deisseroth A, Shen CK, Characterization of an unique RNA initiated immediately upstream from human alpha 1 globin gene in vivo and in vitro: polymerase II- dependence, tissue specificity, and subcellular location. Nucleic Acids Res13:6059-74 1985 |
| PubMed ID: 2413422 |
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| Shipp MA, Schwartz BD, Kannapell CC, Griffith RC, Scott MG, Ahmed P, Davie JM, Nahm MH, A unique DR-related B cell differentiation antigen. J Immunol131:2458-67 1983 |
| PubMed ID: 6415171 |
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| Machado EA, Mitchell JR, Lozzio BB, Lozzio CB, Gerard DA, Absence of morphological, chromosomal and antigenic changes in the K- 562 cell line growing as localized or disseminated tumours in nude mice. Br J Cancer46:383-91 1982 |
| PubMed ID: 6957238 |
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| Lozzio BB, Lozzio CB, Bamberger EG, Feliu AS, A multipotential leukemia cell line (K-562) of human origin. Proc Soc Exp Biol Med166:546-50 1981 |
| PubMed ID: 7194480 |
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| Carrel S, Gross N, Salvi S, Rabbit antiserum against Daudi-cell membrane fractions detects cell surface antigens present on subpopulations of human lymphocytes. J Immunol120:1587-93 1978 |
| PubMed ID: 307019 |
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| Lozzio CB, Lozzio BB, Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood45:321-34 1975 |
| PubMed ID: 163658 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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