Description:
DIABETES MELLITUS: MATURITY ONSET DIABETES - 222100 OR 125850
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Other Disorders of Known Biochemistry |
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Cell Type
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Fibroblast
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|
Transformant
|
Untransformed
|
|
Race
|
White
|
|
Family Member
|
1
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|
Relation to Proband
|
proband
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|
Confirmation
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Clinical summary/Case history
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|
Species
|
Homo sapiens
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|
Common Name
|
Human
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|
Remarks
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|
| Passage Frozen |
3 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
| |
| Remarks |
Third generation of MOD; see GM01241A Lymphoid |
| Burrow JW, Koch JA, Chuang HH, Zhong W, Dean DD, Sylvia VL, Nitric oxide donors selectively reduce the expression of matrix metalloproteinases-8 and -9 by human diabetic skin fibroblasts The Journal of surgical research140:90-8 2006 |
| PubMed ID: 17418871 |
| Passage Frozen |
3 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|