GM01232
Tumor-derived cell line from Tumor
Description:
RETINOBLASTOMA; RB1 OSTEOSARCOMA, RETINOBLASTOMA-RELATED, INCLUDED
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
Hereditary Cancers |
| Class |
Ophthalmologic Disorders |
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Cell Type
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Tumor-derived cell line
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Tissue Type
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Tumor
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Transformant
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Untransformed
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Sample Source
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Tumor-derived cell line from Tumor
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| MORPHOLOGIC DIFFERENTIATION IN VITRO |
Kato and Wakabayashi (In Vitro Cell Devel Biol 24:274-280,1988) studied the responses of this human retinoblastoma cell line to substratum-bound laminin, fibronectin, or collagen in serum containing medium. In this study, the morphologic differentiation of GM01232 cells was induced specifically by laminin bound to poly-D-lysine pretreated plastic surface substratum. This effect was not observed for fibronectin, collagen, or poly-D-lysine alone. Dibutyryl cyclic AMP maintained and promoted the differentiated status and at the same time inhibited cell growth. |
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| Remarks |
Y79; tumor tissue culture, grows in suspension; passage 9 at CCR |
| Conway RM, Madigan MC, King NJ, Billson FA, Penfold PL, Human retinoblastoma: in vitro differentiation and immunoglobulin superfamily antigen modulation by retinoic acid. Cancer Immunol Immunother44:189-96 1997 |
| PubMed ID: 9222276 |
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| Kato G, Wakabayashi K, Effect of polylysine-bound laminin on human retinoblastoma cell lines. In Vitro Cell Dev Biol24:274-80 1988 |
| PubMed ID: 2835353 |
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| Gilbert F, Balaban G, Breg WR, Gallie B, Reid T, Nichols W, Homogeneously staining region in a retinoblastoma cell line: relevance to tumor initiation and progression. J Natl Cancer Inst67:301-306 1981 |
| PubMed ID: 6167750 |
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| Kennett RH, Gilbert F, Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain. Science203:1120-1 1979 |
| PubMed ID: 424740 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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