GM01125
Fibroblast from Cornea, Cornea
Description:
MACULAR DYSTROPHY, CORNEAL, 1; MCDC1
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Ophthalmologic Disorders |
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Biopsy Source
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Cornea
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Cell Type
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Fibroblast
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Tissue Type
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Cornea
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Cornea, Cornea
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
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| Remarks |
Corneal button fibroblast culture; mucopolysaccharidosis type unknown |
| Klintworth GK, Hawkins HK, Smith CF, Acridine orange particles in cultured fibroblasts. A comparative study of macular corneal dystrophy, systemic mucopolysaccharidoses types I-H and II, and normal controls. Arch Pathol Lab Med103:297-9 1979 |
| PubMed ID: 88211 |
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| Klintworth GK, Smith CF, Macular corneal dystrophy. Studies of sulfated glycosaminoglycans in corneal explant and confluent stromal cell cultures. Am J Pathol89:167-82 1977 |
| PubMed ID: 143892 |
| Passage Frozen |
3 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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