GM27730
iPSC from Fibroblast
Description:
PERSONAL GENOME PROJECT
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection
PIGI Consented Sample |
| Protocols |
Protocol PDF |
|
Cell Type
|
Stem cell
|
|
Cell Subtype
|
Induced pluripotent stem cell
|
|
Transformant
|
Reprogrammed (Sendai)
|
|
Sample Source
|
iPSC from Fibroblast
|
|
Race
|
White
|
|
Country of Origin
|
USA
|
|
Family Member
|
1
|
|
Family History
|
N
|
|
Relation to Proband
|
proband
|
|
ISCN
|
46,XY[20].arr(1-22)x2,(X,Y)x1
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| Passage Frozen |
16 |
| |
| Induced Pluripotent Stem Cell |
The parental cell line was recovered, reprogrammed to an induced pluripotent stem cell line, and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
| |
| Remarks |
Participant (huAA53E0) in the Personal Genome Project: http://www.personalgenomes.org
history of Blue rubber bleb nevus syndrome; central serous chorioretinopathy; cystoid macular degeneration; hemangioma; migraine with aura; narcolepsy; sleep paralysis; same subject as GM24385 (lymph) and GM26105 (stem cell from LCL); mother is GM24143 (Lymph) and GM26077 (stem cell); father is GM24149 (Lymph). Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is Sendai-CytoTune. |
| Zhao S, Sinson JC, Li S, Rosenfeld JA, Zapata G, Macakova K, Pena M, Maywald B, Worley KC, Burrage L, Hubshman MW, Ketkar S, Craigen W, Emrick L, Undiagnosed Diseases Network L, Clark T, Lithwick GY, Shipony Z, Eng C, Lee B, Liu P, The Utility of Ultra-Deep RNA sequencing in Mendelian Disorder Diagnostics medRxiv : the preprint server for health sciences: 2025 |
| PubMed ID: 39974001 |
| |
| Caballero M, Ge T, Rebelo AR, Seo S, Kim S, Brooks K, Zuccaro M, Kanagaraj R, Vershkov D, Kim D, Smogorzewska A, Smolka M, Benvenisty N, West SC, Egli D, Mace EM, Koren A, Comprehensive analysis of DNA replication timing across 184 cell lines suggests a role for MCM10 in replication timing regulation Human molecular genetics: 2022 |
| PubMed ID: 35394024 |
| Passage Frozen |
16 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
20% Knock-out Serum Replacement Not inactivated |
| Substrate |
Gelatin + Feeder Layer |
| Supplement |
Basic Fibroblast Growth Factor 10ng/ml |
|