GM23338
iPSC from Fibroblast
Description:
PERSONAL GENOME PROJECT
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection
PIGI Consented Sample |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Retroviral)
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Sample Source
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iPSC from Fibroblast
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Race
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White
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Country of Origin
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USA
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY[20]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
34 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remarks |
Induced pluripotent stem cell line; Participant #1 (hu43860C) in the Personal Genome Project: http://www.personalgenomes.org
Same subject as GM23248 (Fibroblast) and GM20431 (lymphoblast). The original expansion of Parent Fibroblast (GM23248) line is mosaic for t(8;14)(p21.3;q24.3) which is no longer present in the stem cell. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc.. |
| Ewoldt JK, Wang MC, McLellan MA, Cloonan PE, Chopra A, Gorham J, Li L, DeLaughter DM, Gao X, Lee JH, Willcox JAL, Layton O, Luu RJ, Toepfer CN, Eyckmans J, Seidman CE, Seidman JG, Chen CS, Hypertrophic cardiomyopathy-associated mutations drive stromal activation via EGFR-mediated paracrine signaling Science advances10:eadi6927 2024 |
| PubMed ID: 39413182 |
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| Halder SS, Rynkiewicz MJ, Kim L, Barry ME, Zied AG, Sewanan LR, Kirk JA, Moore JR, Lehman WJ, Campbell SG, Distinct mechanisms drive divergent phenotypes in hypertrophic and dilated cardiomyopathy-associated TPM1 variants The Journal of clinical investigation134:eadi6927 2024 |
| PubMed ID: 39436707 |
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| Kobeissi H, Gao X, DePalma SJ, Ewoldt JK, Wang MC, Das SL, Jilberto J, Nordsletten D, Baker BM, Chen CS, Lejeune E, MicroBundlePillarTrack: A Python package for automated segmentation, tracking, and analysis of pillar deflection in cardiac microbundles microPublication biology2024:eadi6927 2024 |
| PubMed ID: 39114859 |
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| Ulicevic J, Shao Z, Jasnovidova O, Bressin A, Gajos M, Ng AH, Annaldasula S, Meierhofer D, Church GM, Busskamp V, Mayer A, Uncovering the dynamics and consequences of RNA isoform changes during neuronal differentiation Molecular systems biology2024:eadi6927 2023 |
| PubMed ID: 38755290 |
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| Jagnandan N, Morachis J, Microfluidic cell sorter sample preparation for genomic assays Biomicrofluidics16:034106 2022 |
| PubMed ID: 35698630 |
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| Alquezar C, Schoch KM, Geier EG, Ramos EM, Scrivo A, Li KH, Argouarch AR, Mlynarski EE, Dombroski B, DeTure M, Dickson DW, Yokoyama JS, Cuervo AM, Burlingame AL, Schellenberg GD, Miller TM, Miller BL, Kao AW, TSC1 loss increases risk for tauopathy by inducing tau acetylation and preventing tau clearance via chaperone-mediated autophagy Science advances7:eabg3897 2021 |
| PubMed ID: 34739309 |
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| Chen DY, Khan N, Close BJ, Goel RK, Blum B, Tavares AH, Kenney D, Conway HL, Ewoldt JK, Chitalia VC, Crossland NA, Chen CS, Kotton DN, Baker SC, Fuchs SY, Connor JH, Douam F, Emili A, Saeed M, SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway Journal of virology7:JVI0086221 2021 |
| PubMed ID: 34260266 |
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| Jo J, Yang L, Tran HD, Yu W, Sun AX, Chang YY, Jung BC, Lee SJ, Saw TY, Xiao B, Khoo ATT, Yaw LP, Xie JJ, Lokman H, Ong WY, Lim GGY, Lim KL, Tan EK, Ng HH, Je HS, Lewy Body-like Inclusions in Human Midbrain Organoids Carrying Glucocerebrosidase and a-Synuclein Mutations Annals of neurology7:JVI0086221 2021 |
| PubMed ID: 34288055 |
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| Shen S, Sewanan LR, Campbell SG, Evidence for synergy between sarcomeres and fibroblasts in an in vitro model of myocardial reverse remodeling Journal of molecular and cellular cardiology158:11-25 2021 |
| PubMed ID: 33992697 |
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| Shen S, Sewanan LR, Shao S, Halder SS, Stankey P, Li X, Campbell SG, Physiological calcium combined with electrical pacing accelerates maturation of human engineered heart tissue Stem cell reports17:2037-2049 2021 |
| PubMed ID: 35931080 |
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| Tian S, Liu Y, Appleton E, Wang H, Church GM, Dong M, Targeted intracellular delivery of Cas13 and Cas9 nucleases using bacterial toxin-based platforms Cell reports38:110476 2021 |
| PubMed ID: 35263584 |
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| Jung S, Appleton E, Ali M, Church GM, Del Sol A, A computer-guided design tool to increase the efficiency of cellular conversions Nature communications12:1659 2020 |
| PubMed ID: 33712564 |
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| Ladha FA, Thakar K, Pettinato AM, Legere N, Ghahremani S, Cohn R, Romano R, Meredith E, Chen YS, Hinson JT, Actinin BioID reveals sarcomere crosstalk with oxidative metabolism through interactions with IGF2BP2 Cell reports36:109512 2020 |
| PubMed ID: 34380038 |
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| Ng R, Sewanan LR, Brill AL, Stankey P, Li X, Yibing Qyang YQ, Ehrlich BE, Campbell SG, Contractile work directly modulates mitochondrial protein levels in human engineered heart tissues American journal of physiology Heart and circulatory physiology36:109512 2020 |
| PubMed ID: 32383992 |
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| Pettinato AM, Yoo D, VanOudenhove J, Chen YS, Cohn R, Ladha FA, Yang X, Thakar K, Romano R, Legere N, Meredith E, Robson P, Regnier M, Cotney JL, Murry CE, Hinson JT, Sarcomere function activates a p53-dependent DNA damage response that promotes polyploidization and limits in vivo cell engraftment Cell reports35:109088 2020 |
| PubMed ID: 33951429 |
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| Venkatesh S, Baljinnyam E, Tong M, Kashihara T, Yan L, Liu T, Li H, Xie LH, Nakamura M, Oka SI, Suzuki CK, Fraidenraich D, Sadoshima J, Proteomic analysis of mitochondrial biogenesis in cardiomyocytes differentiated from human induced pluripotent stem cells American journal of physiology Regulatory, integrative and comparative physiology35:109088 2020 |
| PubMed ID: 33112656 |
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| Brill AL, Fischer TT, Walters JM, Marlier A, Sewanan LR, Wilson PC, Johnson EK, Moeckel G, Cantley LG, Campbell SG, Nerbonne JM, Chung HJ, Robert ME, Ehrlich BE, Polycystin 2 is increased in disease to protect against stress-induced cell death Scientific reports10:386 2019 |
| PubMed ID: 31941974 |
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| Larsen SB, Hanss Z, Cruciani G, Massart F, Barbuti PA, Mellick G, Krüger R, Induced pluripotent stem cell line (LCSBi001-A) derived from a patient with Parkinson's disease carrying the pD620N mutation in VPS35 Stem cell research45:101776 2019 |
| PubMed ID: 32387898 |
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| Ng R, Manring H, Papoutsidakis N, Albertelli T, Tsai N, See CJ, Li X, Park J, Stevens TL, Bobbili PJ, Riaz M, Ren Y, Stoddard CE, Janssen PM, Bunch TJ, Hall SP, Lo YC, Jacoby DL, Qyang Y, Wright N, Ackermann MA, Campbell SG, Patient mutations linked to arrhythmogenic cardiomyopathy enhance calpain-mediated desmoplakin degradation JCI insight5:101776 2019 |
| PubMed ID: 31194698 |
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| Sauter EJ, Kutsche LK, Klapper SD, Busskamp V, Induced Neurons for the Study of Neurodegenerative and Neurodevelopmental Disorders Methods in molecular biology (Clifton, NJ)1942:101-121 2019 |
| PubMed ID: 30900179 |
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| Cohn R, Thakar K, Lowe A, Ladha FA, Pettinato AM, Romano R, Meredith E, Chen YS, Atamanuk K, Huey BD, Hinson JT, A Contraction Stress Model of Hypertrophic Cardiomyopathy due to Sarcomere Mutations Stem Cell Reports1942:101-121 2018 |
| PubMed ID: 30554920 |
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| Walter J, Bolognin S, Antony PMA, Nickels SL, Poovathingal SK, Salamanca L, Magni S, Perfeito R, Hoel F, Qing X, Jarazo J, Arias-Fuenzalida J, Ignac T, Monzel AS, Gonzalez-Cano L, Pereira de Almeida L, Skupin A, Tronstad KJ, Schwamborn JC, Neural Stem Cells of Parkinson's Disease Patients Exhibit Aberrant Mitochondrial Morphology and Functionality Stem cell reports12:878-889 2018 |
| PubMed ID: 30982740 |
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| Colasuonno F, Borghi R, Niceforo A, Muzzi M, Bertini E, Di Giulio A, Moreno S, Compagnucci C, Senescence-associated ultrastructural features of long-term cultures of induced pluripotent stem cells (iPSCs) Aging12:878-889 2017 |
| PubMed ID: 29064821 |
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| Drouin-Ouellet J, Pircs K, Barker RA, Jakobsson J, Parmar M, Direct Neuronal Reprogramming for Disease Modeling Studies Using Patient-Derived Neurons: What Have We Learned? Frontiers in neuroscience11:530 2017 |
| PubMed ID: 29033781 |
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| Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016 |
| PubMed ID: 27724973 |
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| Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005 |
| PubMed ID: 16729065 |
| Passage Frozen |
34 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none Not inactivated |
| Substrate |
Matrigel |
| Supplement |
- |
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