GM23279
iPSC from Fibroblast
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Retroviral)
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Sample Source
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iPSC from Fibroblast
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Race
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White
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX[20]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
31 |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remarks |
Induced pluripotent stem cell line made from facial dermal fibroblasts; retroviruses used contained human Oct3/4, Sox2, Klf4, and c-Myc. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc..
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| N Costa M, Goto-Silva L, M Nascimento J, Domith I, Karmirian K, Feilding A, Trindade P, Martins-de-Souza D, K Rehen S, LSD Modulates Proteins Involved in Cell Proteostasis, Energy Metabolism and Neuroplasticity in Human Cerebral Organoids ACS omega9:36553-36568 2024 |
| PubMed ID: 39220485 |
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| Nugue G, Martins M, Vitória G, Guimaraes BLML, Quiñones-Vega M, Rehen S, Guimarães MZ, Junqueira M, Optimized pipeline for personalized neurobiological insights from single patient-derived Neurospheres Journal of proteomics9:105368 2024 |
| PubMed ID: 39657900 |
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| Souza LRQ, Pedrosa CGDS, Puig-Pijuan T, da Silva Dos Santos C, Vitória G, Delou JMA, Setti-Perdigão P, Higa LM, Tanuri A, Rehen SK, Guimarães MZP, Saxitoxin potentiates human neuronal cell death induced by Zika virus while sparing neural progenitors and astrocytes Scientific reports14:22809 2024 |
| PubMed ID: 39354036 |
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| Carregari VC, Reis-de-Oliveira G, Crunfli F, Smith BJ, de Souza GF, Muraro SP, Saia-Cereda VM, Vendramini PH, Baldasso PA, Silva-Costa LC, Zuccoli GS, Brandão-Teles C, Antunes A, Valença AF, Davanzo GG, Virgillio-da-Silva JV, Dos Reis Araújo T, Guimarães RC, Chaim FDM, Chaim EA, Kawagosi Onodera CM, Ludwig RG, Saccon TD, Damásio ARL, Leiria LOS, Vinolo MAR, Farias AS, Moraes-Vieira PM, Mori MA, Módena JLP, Martins-de-Souza D, Diving into the proteomic atlas of SARS-CoV-2 infected cells Scientific reports14:7375 2023 |
| PubMed ID: 38548777 |
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| Hawkins S, Mondaini A, Namboori SC, Nguyen GG, Yeo GW, Javed A, Bhinge A, ePRINT: exonuclease assisted mapping of protein-RNA interactions Genome biology25:140 2023 |
| PubMed ID: 38807229 |
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| Shen WB, Logue J, Yang P, Baracco L, Elahi M, Reece EA, Wang B, Li L, Blanchard TG, Han Z, Frieman MB, Rissman RA, Yang P, SARS-CoV-2 invades cognitive centers of the brain and induces Alzheimer's-like neuropathology bioRxiv : the preprint server for biology25:140 2022 |
| PubMed ID: 35132414 |
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| Assis-de-Lemos G, Monteiro J, Oliveira-Valença VM, Melo GA, Reis RAM, Rehen SK, Silveira MS, Galina A, Dopamine signaling impairs ROS modulation by mitochondrial hexokinase in human neural progenitor cells Bioscience reports41:140 2021 |
| PubMed ID: 34821365 |
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| Notaras M, Lodhi A, Barrio-Alonso E, Foord C, Rodrick T, Jones D, Fang H, Greening D, Colak D, Neurodevelopmental signatures of narcotic and neuropsychiatric risk factors in 3D human-derived forebrain organoids Molecular psychiatry41:140 2021 |
| PubMed ID: 34158620 |
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| Notaras M, Lodhi A, Dündar F, Collier P, Sayles NM, Tilgner H, Greening D, Colak D, Schizophrenia is defined by cell-specific neuropathology and multiple neurodevelopmental mechanisms in patient-derived cerebral organoids Molecular psychiatry41:140 2021 |
| PubMed ID: 34789849 |
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| Notaras M, Lodhi A, Fang H, Greening D, Colak D, The proteomic architecture of schizophrenia iPSC-derived cerebral organoids reveals alterations in GWAS and neuronal development factors Translational psychiatry11:541 2021 |
| PubMed ID: 34667143 |
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| Ornelas IM, Cini FA, Wießner I, Marcos E, Araújo DB, Goto-Silva L, Nascimento J, Silva SRB, Costa MN, Falchi M, Olivieri R, Palhano-Fontes F, Sequerra E, Martins-de-Souza D, Feilding A, Rennó-Costa C, Tófoli LF, Rehen SK, Ribeiro S, Nootropic effects of LSD: Behavioral, molecular and computational evidence Experimental neurology356:114148 2021 |
| PubMed ID: 35732217 |
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| Pamplona FA, Vitória G, Sudo FK, Ribeiro FC, Isaac AR, Moraes CA, Chauvet MG, Ledur PF, Karmirian K, Ornelas IM, Leo LM, Paulsen B, Coutinho G, Drummond C, Assunção N, Vanderborght B, Canetti CA, Castro-Faria-Neto HC, Mattos P, Ferreira ST, Rehen SK, Bozza FA, Lourenco MV, Tovar-Moll F, Age-linked suppression of lipoxin A4 associates with cognitive deficits in mice and humans Translational psychiatry12:439 2021 |
| PubMed ID: 36216800 |
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| Delepine C, Pham VA, Tsang HWS, Sur M, GSK3ß inhibitor CHIR 99021 modulates cerebral organoid development through dose-dependent regulation of apoptosis, proliferation, differentiation and migration PloS one16:e0251173 2020 |
| PubMed ID: 33951093 |
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| Khan I, Prabhakar A, Delepine C, Tsang H, Pham V, Sur M, A low-cost 3D printed microfluidic bioreactor and imaging chamber for live-organoid imaging Biomicrofluidics15:024105 2020 |
| PubMed ID: 33868534 |
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| White JA, Krzystek TJ, Hoffmar-Glennon H, Thant C, Zimmerman K, Iacobucci G, Vail J, Thurston L, Rahman S, Gunawardena S, Excess Rab4 rescues synaptic and behavioral dysfunction caused by defective HTT-Rab4 axonal transport in Huntington's disease Acta neuropathologica communications8:97 2020 |
| PubMed ID: 32611447 |
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| Kim E, Wu F, Wu X, Choo HJ, Generation of craniofacial myogenic progenitor cells from human induced pluripotent stem cells for skeletal muscle tissue regeneration Biomaterials248:119995 2019 |
| PubMed ID: 32283390 |
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| Ledur PF, Karmirian K, Pedrosa CDSG, Souza LRQ, Assis-de-Lemos G, Martins TM, Ferreira JCCG, de Azevedo Reis GF, Silva ES, Silva D, Salerno JA, Ornelas IM, Devalle S, Madeiro da Costa RF, Goto-Silva L, Higa LM, Melo A, Tanuri A, Chimelli L, Murata MM, Garcez PP, Filippi-Chiela EC, Galina A, Borges HL, Rehen SK, Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes Scientific reports10:1218 2019 |
| PubMed ID: 31988337 |
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| Tonon LAC, de Azevedo GPR, Monteiro AF, Bernardi DI, Gubiani JR, Ióca LP, Mattsson HK, Moreira APB, Gomes AF, Pires Junior OR, da S G Pedrosa C, Souza LRQ, Rehen SK, Thompson CC, Thompson FL, Berlinck RGS, New tetrodotoxin analogs in Brazilian pufferfishes tissues and microbiome Chemosphere242:125211 2019 |
| PubMed ID: 31896201 |
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| Goto-Silva L, Ayad NME, Herzog IL, Silva NP, Lamien B, Orlande HRB, da Costa Souza A, Ribeiro S, Martins M, Domont GB, Junqueira M, Tovar-Moll F, Rehen SK, Computational fluid dynamic analysis of physical forces playing a role in brain organoid cultures in two different multiplex platforms BMC developmental biology19:3 2018 |
| PubMed ID: 30841924 |
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| Casas BS, Vitória G, do Costa MN, Madeiro da Costa R, Trindade P, Maciel R, Navarrete N, Rehen SK, Palma V, hiPSC-derived neural stem cells from patients with schizophrenia induce an impaired angiogenesis Translational psychiatry8:48 2017 |
| PubMed ID: 29467462 |
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| Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S., Induction of pluripotent stem cells from adult human fibroblasts by defined factors Cell131:861-872 2007 |
| PubMed ID: 18035408 |
| Passage Frozen |
31 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none |
| Substrate |
Matrigel |
| Supplement |
- |
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