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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Chinese hamster lung-derived cell line V79B by Ford and Yerganian (1958) from a young male animal, and originally designated as V cells. This cell line was renamed V79 by Elkind in 1958 and then used widely. Several different subclones emerged such as V79-4 or V79B making it important to use the true parental cell lines as controls for different mutants. V79B cells were obtained from Dr. C. F. Arlett (MRC Cell Mutation Unit, University of Sussex, UK). The morphology of V79B differs from that of V79 cells, and V79B cells are more efficient in stable transfections. Several mutants with increased sensitivity to different genotoxic agents have been isolated from V79B, including XR-V15B (GM16144). |
| Hoeijmakers, J. H., Methods and strategies for molecular cloning of mammalian DNA repair genes by DN-mediated gene transfer in "DNA Repair, Lab. Manual for Research Procedures", Eds. E. C. Friedberg and P. C. Hanawalt; Marcel Dekker Inc., New York 3:181-201 1988 |
| PubMed ID: |
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| Gottesman, M. M., "Molecular Cell Genetics", New York, Wiley 3:66-74 1985 |
| PubMed ID: |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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