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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Chinese hamster lung mutant cell line V-H4, derived from V79 (GM16136) by ethylnitrosourea-induced mutagenesis. V-H4 is hypersensitive to mitomycin C and to other cross-linking agents, and its phenotype resembles that of cells derived from Fanconi anemia individuals. |
| Telleman P, Overkamp WJ, Zdzienicka MZ, Spectrum of spontaneously occurring mutations in the HPRT gene of the Chinese
hamster V79 cell mutant V-H4, which is homologous to Fanconi anemia group A. Mutagenesis11(2):155-9 1996 |
| PubMed ID: 8671732 |
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| Sakaguchi K, Zdzienicka MZ, Harris PV, Boyd JB, Nuclease modification in Chinese hamster cells hypersensitive to DNA
cross-linking agents--a model for Fanconi anemia. Mutat Res274(1):11-8 1992 |
| PubMed ID: 1375328 |
| |
| Arwert F, Rooimans MA, Westerveld A, Simons JW, Zdzienicka MZ, The Chinese hamster cell mutant V-H4 is homologous to Fanconi anemia (complementation group A). Cytogenet Cell Genet56:23-6 1991 |
| PubMed ID: 1900747 |
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| Zdzienicka MZ, Arwert F, Neuteboom I, Rooimans M, Simons JW, The Chinese hamster V79 cell mutant V-H4 is phenotypically like Fanconi anemia
cells. Somat Cell Mol Genet16(6):575-81 1990 |
| PubMed ID: 2267631 |
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| Zdzienicka MZ, Simons JW, Mutagen-sensitive cell lines are obtained with a high frequency in V79 Chinese
hamster cells. Mutat Res178(2):235-44 1987 |
| PubMed ID: 3587254 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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