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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Gonad
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Genbank |
COX1 |
| Remarks |
Chinese hamster ovary cell line EM-C11 was obtained by ethylnitrosourea induced mutagenesis of CHO-9 fibroblast cells (GM16132). It is sensitive to simple alkylating agents and has a 10 fold elevated level of sister chromatid exchanges. EM-C11 is a member of x-ray cross complementation group XRCC1. It is defective in the repair of DNA single strand breaks and in DNA base excision repair. EM-C11 is defective in the XRCC1 gene. In the base excision repair mechanism the product of the XRCC1 gene forms a complex with DNA ligase III. |
| Shen MR, Zdzienicka MZ, Mohrenweiser H, Thompson LH, Thelen MP, Mutations in hamster single-strand break repair gene XRCC1 causing defective DNA
repair. Nucleic Acids Res26(4):1032-7 1998 |
| PubMed ID: 9461464 |
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| Cappelli E, Taylor R, Cevasco M, Abbondandolo A, Caldecott K, Frosina G, Involvement of XRCC1 and DNA ligase III gene products in DNA base excision
repair. J Biol Chem272(38):23970-5 1997 |
| PubMed ID: 9295348 |
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| Caldecott KW, Tucker JD, Stanker LH, Thompson LH, Characterization of the XRCC1-DNA ligase III complex in vitro and its absence
from mutant hamster cells. Nucleic Acids Res23(23):4836-43 1995 |
| PubMed ID: 8532526 |
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| Op het Veld CW, Zdzienicka MZ, Vrieling H, Lohman PH, van Zeeland AA, Molecular analysis of ethyl methanesulfonate-induced mutations at the hprt gene
in the ethyl methanesulfonate-sensitive Chinese hamster cell line EM-C11 and its
parental line CHO9. Cancer Res54(11):3001-6 1994 |
| PubMed ID: 8187089 |
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| Zdzienicka MZ, van der Schans GP, Natarajan AT, Thompson LH, Neuteboom I,
Simons JW, A Chinese hamster ovary cell mutant (EM-C11) with sensitivity to simple
alkylating agents and a very high level of sister chromatid exchanges. Mutagenesis7(4):265-9 1992 |
| PubMed ID: 1518409 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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