GM09852
Fibroblast from Skin, Unspecified
Description:
ICHTHYOSIS, X-LINKED
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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pSV3-neo plasmid
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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White
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Ethnicity
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SARDINIAN
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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Unstable karyotype; near tetraploid; 100% cells show abnormalities; chromosomes per cell varies from 48 to 88
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
25 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis. DNA fingerprinting and HLA DQ-alpha typing showed that this pSV3-neo transformed fibroblast culture had the same pattern and genotype as GM03030, the untransformed fibroblast. |
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| Remarks |
Transformant produced by transfecting GM03030 with DNA from the pSV3-neo plasmid carrying a dominant selection marker; selectable with the antibiotic G418 an aminoglycoside; 100% cells with chromo abnormalities; T-antigen positive |
| Chang PL, Gunby JL, Tomkins DJ, Mak I, Rosa NE, Mak S, Transformation of human cultured fibroblasts with plasmids carrying dominant selection markers and immortalizing potential. Exp Cell Res167:407-16 1986 |
| PubMed ID: 3021482 |
| Passage Frozen |
25 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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