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GM07189 Fibroblast from Fetal, Unspecified

Description:

ANEUPLOID CHROMOSOME NUMBER - TRISOMY

Affected:

No Data

Sex:

Other

Age:

12 FW (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Chromosome Abnormalities
Biopsy Source Unspecified
Cell Type Fibroblast
Tissue Type Fetal
Transformant Untransformed
Sample Source Fibroblast from Fetal, Unspecified
Race White
Relation to Proband proband
Confirmation Karyotypic analysis after cell line submission to CCR
ISCN 47,XY,+15[20]
Species Homo sapiens
Common Name Human
Remarks Fibroblast culture established from fetal membranes

Characterizations

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PDL at Freeze 5.07
Passage Frozen 7
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis
 
Cytogenetics Chromosome 15: ANEUPLOID Aneuploid Segment (+)15pter>15qter
Chromosome 15: ANEUPLOID Trisomic Segment 15pter>15qter

Phenotypic Data

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Remarks Fibroblast culture established from fetal membranes

Publications

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Krivega M, Stiefel CM, Karbassi S, Andersen LL, Chunduri NK, Donnelly N, Pichlmair A, Storchová Z, Genotoxic stress in constitutive trisomies induces autophagy and the innate immune response via the cGAS-STING pathway Communications biology4:831 2021
PubMed ID: 34215848
 
García-Pascual CM, Navarro-Sánchez L, Navarro R, Martínez L, Jiménez J, Rodrigo L, Simón C, Rubio C, Optimized NGS Approach for Detection of Aneuploidies and Mosaicism in PGT-A and Imbalances in PGT-SR Genes11:831 2020
PubMed ID: 32610655
 
Hu DG, Webb G, Hussey N, Aneuploidy detection in single cells using DNA array-based comparative genomic hybridization Molecular human reproduction10:283-9 2004
PubMed ID: 14985479

External Links

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dbSNP dbSNP ID: 21026

Culture Protocols

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Passage Frozen 7
Split Ratio 1:2
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Supplement -
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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How to Order
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  • Statement of Research Intent Form
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