GM05868
Fibroblast from Skin, Unspecified
Description:
BOVINE PROTOPORPHYRIA
BOS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Bos taurus
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Common Name
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cow
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Remarks
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| Passage Frozen |
8 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
| |
| Remarks |
Fibroblast culture from a 6 year old female 1/2 Limousin 1/2 Hereford; clinically normal carrier for bovine protoporphyria |
| Ruth, A new disease of cattle - bovine protoporphyria, clinical and diagnostic features. Am Assn Vet Lab Diagn 21st Ann Procp91 (1978): 1978 |
| PubMed ID: |
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| RUTH GR, Schwartz S, Stephenson B, Bovine protoporphyria: the first nonhuman model of this hereditary photosensitizing disease. Science198:199-201 1977 |
| PubMed ID: 905823 |
| Passage Frozen |
8 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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