GM04672
LCL from B-Lymphocyte
Description:
MYELOMA, MULTIPLE AMYLOIDOSIS, PRIMARY, INCLUDED; AL, INCLUDED
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Other Disorders of Known Biochemistry |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Family Member
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1
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
EMS induced mutant of GM01500; HPRT deficient; selectable with 6-thioguanine; secretes IgG2 (Kappa); has been used to make human-human hybridomas; line GM1500 6TG-A1 (Croce) |
| Meng QH, Rauch J, Differences between human hybridoma platelet-binding antibodies derived from systemic lupus erythematosus patients and normal individuals. Autoimmunity5:151-67 1990 |
| PubMed ID: 2129749 |
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| Newkirk MM, Lemmo A, Rauch J, Importance of the IgG isotype, not the state of glycosylation, in determining human rheumatoid factor binding. Arthritis Rheum33:800-9 1990 |
| PubMed ID: 2363735 |
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| Ohtani K, Nakamura M, Saito S, Nagata K, Sugamura K, Hinuma Y, Electroporation: application to human lymphoid cell lines for stable introduction of a transactivator gene of human T-cell leukemia virus type I. Nucleic Acids Res17:1589-604 1989 |
| PubMed ID: 2784206 |
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| Senecal JL, Rauch J, Hybridoma lupus autoantibodies can bind major cytoskeletal filaments in the absence of DNA-binding activity. Arthritis Rheum31:864-75 1988 |
| PubMed ID: 3293572 |
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| Kozbor D, Burioni R, Ar-Rushdi A, Zmijewski C, Croce CM, Expression of members of immunoglobulin gene family in somatic cell hybrids between human B and T cells. Proc Natl Acad Sci U S A84:4969-73 1987 |
| PubMed ID: 3496599 |
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| Rauch J, Meng QH, Tannenbaum H, Lupus anticoagulant and antiplatelet properties of human hybridoma autoantibodies. J Immunol139:2598-604 1987 |
| PubMed ID: 3116088 |
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| Cairns E, Komar R, Bell DA, Cytoskeletal binding of monoclonal anti-DNA antibodies derived from tonsillar lymphoid cells of a normal subject. Arthritis Rheum29:1351-8 1986 |
| PubMed ID: 3535810 |
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| Cote RJ, Morrissey DM, Houghton AN, Thomson TM, Daly ME, Oettgen HF, Old LJ, Specificity analysis of human monoclonal antibodies reactive with cell surface and intracellular antigens. Proc Natl Acad Sci U S A83:2959-63 1986 |
| PubMed ID: 3458255 |
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| Littlefield BA, Hoagland HC, Greipp PR, Elevated glucocorticoid receptor binding in cultured human lymphoblasts following hydroxyurea treatment: lack of effect on steroid responsiveness. Cancer Res46:3945-50 1986 |
| PubMed ID: 3731065 |
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| Rauch J, Tannenbaum H, Straaton K, Massicotte H, Wild J, Human-human hybridoma autoantibodies with both anti-DNA and rheumatoid factor activities. J Clin Invest77:106-12 1986 |
| PubMed ID: 3511088 |
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| Rauch J, Tannenbaum M, Tannenbaum H, Ramelson H, Cullis PR, Tilcock CP, Hope MJ, Janoff AS, Human hybridoma lupus anticoagulants distinguish between lamellar and hexagonal phase lipid systems. J Biol Chem261:9672-7 1986 |
| PubMed ID: 3733693 |
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| Schmidt-Ullrich R, Brown J, Whittle H, Lin PS, Human-human hybridomas secreting monoclonal antibodies to the Mr 195,000 Plasmodium falciparum blood stage antigen. J Exp Med163:179-88 1986 |
| PubMed ID: 2416867 |
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| Rauch J, Massicotte H, Tannenbaum H, Hybridoma anti-DNA autoantibodies from patients with rheumatoid arthritis and systemic lupus erythematosus demonstrate similar nucleic acid binding characteristics. J Immunol134:180-6 1985 |
| PubMed ID: 3964813 |
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| Rauch J, Massicotte H, Tannenbaum H, Specific and shared idiotypes found on hybridoma anti-DNA autoantibodies derived from rheumatoid arthritis and systemic lupus erythematosus patients. J Immunol135:2385-92 1985 |
| PubMed ID: 3875652 |
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| Behrens UJ, Paronetto F, Elimination of the yeast Candida parapsilosis from lymphoid cells and monolayer cells in culture. In Vitro20:391-5 1984 |
| PubMed ID: 6586685 |
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| Cairns E, Block J, Bell DA, Anti-DNA autoantibody-producing hybridomas of normal human lymphoid cell origin. J Clin Invest74:880-7 1984 |
| PubMed ID: 6470143 |
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| Croce CM, Erikson J, ar-Rushdi A, Aden D, Nishikura K, Translocated c-myc oncogene of Burkitt lymphoma is transcribed in plasma cells and repressed in lymphoblastoid cells. Proc Natl Acad Sci U S A81:3170-4 1984 |
| PubMed ID: 6328505 |
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| Massicotte H, Rauch J, Shoenfeld Y, Tannenbaum H, Influence of fusion cell ratio and cell plating density on production of human-human hybridomas secreting anti-DNA autoantibodies from patients with systemic lupus erythematosus. Hybridoma3:215-22 1984 |
| PubMed ID: 6500583 |
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| Normansell DE, Lane JR, In vitro production of monoclonal human rheumatoid factors. Ann Clin Lab Sci14:64-8 1984 |
| PubMed ID: 6320710 |
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| Taves CJ, Kusumi A, Winkelhake JL, Human aglycosyl-IgG exhibits increased hydrophobicity. Binding/fluorescence studies with 8-anilinonaphthalene-1-sulfonic acid (ANS). Biochem Biophys Res Commun124:605-13 1984 |
| PubMed ID: 6497893 |
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| Shoenfeld Y, Isenberg DA, Rauch J, Madaio MP, Stollar BD, Schwartz RS, Idiotypic cross-reactions of monoclonal human lupus autoantibodies. J Exp Med158:718-30 1983 |
| PubMed ID: 6886627 |
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| Shoenfeld Y, Rauch J, Massicotte H, Datta SK, Andre-Schwartz J, Stollar BD, Schwartz RS, Polyspecificity of monoclonal lupus autoantibodies produced by human- human hybridomas. N Engl J Med308:414-20 1983 |
| PubMed ID: 6823251 |
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| Shoenfeld Y, Hsu-Lin SC, Gabriels JE, Silberstein LE, Furie BC, Furie B, Stollar BD, Schwartz RS, Production of autoantibodies by human-human hybridomas. J Clin Invest70:205-8 1982 |
| PubMed ID: 7085884 |
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| Croce CM, Linnenbach A, Hall W, Steplewski Z, Koprowski H, Production of human hybridomas secreting antibodies to measles virus. Nature288:488-9 1980 |
| PubMed ID: 6969366 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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