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GM04155 LCL from B-Lymphocyte

Description:

ACUTE LYMPHOCYTIC LEUKEMIA
LYMPHOSARCOMA

Affected:

Yes

Sex:

Male

Age:

11 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Heritable Diseases
Class Heritable Cancer Syndromes and other Cancers
Biopsy Source Peripheral vein
Cell Type T-Lymphocyte
Tissue Type Blood
Transformant Possibly Human T-cell Leukemia Virus
Sample Source LCL from B-Lymphocyte
Race White
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
ISCN XY
Species Homo sapiens
Common Name Human
Remarks T cell line CCRF-HSB2; same patient as GM04154B; negative for T cell 65,000 MW glycoprotein, sIg, & Ia(B cell) antigen; all cells nearly diploid with frequent chromosome loss; marker chromosome (13q-?) present in all cells

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Chromosome Analysis
 

Phenotypic Data

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Remarks T cell line CCRF-HSB2; same patient as GM04154B; negative for T cell 65,000 MW glycoprotein, sIg, & Ia(B cell) antigen; all cells nearly diploid with frequent chromosome loss; marker chromosome (13q-?) present in all cells

Publications

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Haataja L, Groffen J, Heisterkamp N, Characterization of RAC3, a novel member of the Rho family. J Biol Chem272:20384-8 1997
PubMed ID: 9252344
 
Heisterkamp N, Mulder MP, Langeveld A, ten Hoeve J, Wang Z, Roe BA, Groffen J, Localization of the human mitochondrial citrate transporter protein gene to chromosome 22Q11 in the DiGeorge syndrome critical region. Genomics29:451-6 1995
PubMed ID: 8666394
 
Wellner VP, Anderson ME, Puri RN, Jensen GL, Meister A, Radioprotection by glutathione ester: transport of glutathione ester into human lymphoid cells and fibroblasts. Proc Natl Acad Sci U S A81:4732-5 1984
PubMed ID: 6146978
 
Shipp MA, Schwartz BD, Kannapell CC, Griffith RC, Scott MG, Ahmed P, Davie JM, Nahm MH, A unique DR-related B cell differentiation antigen. J Immunol131:2458-67 1983
PubMed ID: 6415171
 
Daddona PE, Human adenosine deaminase. Properties and turnover in cultured T and B lymphoblasts. J Biol Chem256:12496-501 1981
PubMed ID: 6975278
 
Carrel S, Gross N, Salvi S, Rabbit antiserum against Daudi-cell membrane fractions detects cell surface antigens present on subpopulations of human lymphocytes. J Immunol120:1587-93 1978
PubMed ID: 307019
 
Lazarus H, Barell EF, Oppenheim S, Krishan A, Divergent properties of two human lymphocytic cell lines isolated from a single specimen of peripheral blood. In Vitro9:303-10 1974
PubMed ID: 4523807
 
Adams RA, Flowers A, Davis BJ, Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res28:1121-5 1968
PubMed ID: 4872716

External Links

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dbSNP dbSNP ID: 13617
Gene Cards TAL1
Gene Ontology GO:0003677 DNA binding
GO:0006355 regulation of transcription, DNA-dependent
GO:0008283 cell proliferation
GO:0030154 cell differentiation
NCBI Gene Gene ID:6886
NCBI GTR 187040 T-CELL ACUTE LYMPHOCYTIC LEUKEMIA 1; TAL1
OMIM 187040 T-CELL ACUTE LYMPHOCYTIC LEUKEMIA 1; TAL1
Omim Description STEM CELL LEUKEMIA HEMATOPOIETIC TRANSCRIPTION FACTOR; SCL
  T-CELL ACUTE LYMPHOCYTIC LEUKEMIA 1; TAL1
  T-CELL LEUKEMIA/LYMPHOMA 5; TCL5, FORMERLY

Culture Protocols

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Split Ratio 1:4
Temperature 37 C
Percent CO2 5%
Medium Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent
Serum 20% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method dilution - add fresh medium
Supplement -
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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