GM03639
LCL from B-Lymphocyte
Description:
ACUTE LYMPHOCYTIC LEUKEMIA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Heritable Cancer Syndromes and other Cancers |
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Biopsy Source
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Peripheral vein
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Cell Type
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T-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Possibly Human T-cell Leukemia Virus
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Sample Source
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LCL from B-Lymphocyte
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
T cell line 8402; same patient as GM03638C; positive for T cell 65,000 MW glycoprotein and negative for sIg and Ia (B cell) antigen; all cells show 6q- and 11p+; 18% are near diploid, 72% are hypotetraploid, and all show random chromosome abnormalities |
| Cho J, Lee Y, Lutzky J, Redpath L, Slater L, Collateral sensitivity to radiation and cis-platinum in a multidrug-resistant human leukemia cell line. Cancer Chemother Pharmacol37:168-72 1995 |
| PubMed ID: 7497588 |
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| Beland JL, Yuille MA, Hugunin M, Zhang XM, Silverstone AE, Phorbol ester regulation of terminal deoxynucleotidyl transferase, proliferation, and TcR alpha in a pre-T cell line. J Cell Physiol142:523-32 1990 |
| PubMed ID: 2138160 |
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| Gan TE, Dadonna PE, Mitchell BS, Genetic expression of adenosine deaminase in human lymphoid malignancies. Blood69:1376-80 1987 |
| PubMed ID: 3567360 |
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| Lebo RV, Anderson LA, Lau YF, Flandermeyer R, Kan YW, Flow-sorting analysis of normal and abnormal human genomes. Cold Spring Harb Symp Quant Biol51 Pt 1:169-76 1986 |
| PubMed ID: 3472713 |
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| Lebo RV, Golbus MS, Cheung MC, Detecting abnormal human chromosome constitutions by dual laser flow cytogenetics. Am J Med Genet25:519-29 1986 |
| PubMed ID: 2431619 |
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| Verhoef V, Fridland A, Metabolic basis of arabinonucleoside selectivity for human leukemic T- and B-lymphoblasts. Cancer Res45:3646-50 1985 |
| PubMed ID: 2410098 |
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| Behrens UJ, Paronetto F, Elimination of the yeast Candida parapsilosis from lymphoid cells and monolayer cells in culture. In Vitro20:391-5 1984 |
| PubMed ID: 6586685 |
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| Slater, Verapamil (Vp) enhances vincristine (VCR ) cytotoxicity in human acute lymphatic leukemia (ALL) in long term tissue culture. Proc Am Assoc Cancer ResAACR Abs #1340, 1984:391-5 1984 |
| PubMed ID: 6586685 |
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| Daddona PE, Human adenosine deaminase. Properties and turnover in cultured T and B lymphoblasts. J Biol Chem256:12496-501 1981 |
| PubMed ID: 6975278 |
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| Omary MB, Trowbridge IS, Covalent binding of fatty acid to the transferrin receptor in cultured human cells. J Biol Chem256:4715-8 1981 |
| PubMed ID: 6262300 |
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| Royston I, Majda JA, Baird SM, Meserve BL, Griffiths JC, Human T cell antigens defined by monoclonal antibodies: the 65,000- dalton antigen of T cells (T65) is also found on chronic lymphocytic leukemia cells bearing surface immunoglobulin. J Immunol125:725-31 1980 |
| PubMed ID: 6993560 |
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| Huang CC, Hou Y, Woods LK, Moore GE, Minowada J, Cytogenetic study of human lymphoid T-cell lines derived from lymphocytic leukemia. J Natl Cancer Inst53:655-660 1974 |
| PubMed ID: 4547172 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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