GM02219
LCL from B-Lymphocyte
Description:
ACUTE LYMPHOCYTIC LEUKEMIA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
dbGaP |
| Class |
Heritable Cancer Syndromes and other Cancers |
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Biopsy Source
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Peripheral vein
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Cell Type
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T-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Possibly Human T-cell Leukemia Virus
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Sample Source
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LCL from B-Lymphocyte
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line Molt-4; T lymphoblast cell line; all cells have 1p-, 6q-, 7p+, and an unknown marker chromosome; line is tetraploid; culture is positive for T cell 65,000 MW glycoprotein and negative for sIg and Ia (B cell) antigen |
| Cheng S, Grow MA, Pallaud C, Klitz W, Erlich HA, Visvikis S, Chen JJ, Pullinger CR, Malloy MJ, Siest G, Kane JP, A multilocus genotyping assay for candidate markers of cardiovascular disease risk. Genome Res9:936-49 1999 |
| PubMed ID: 10523522 |
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| Lattier DL, States JC, Hutton JJ, Wiginton DA, Cell type-specific transcriptional regulation of the human adenosine deaminase gene. Nucleic Acids Res17:1061-76 1989 |
| PubMed ID: 2784203 |
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| Gan TE, Dadonna PE, Mitchell BS, Genetic expression of adenosine deaminase in human lymphoid malignancies. Blood69:1376-80 1987 |
| PubMed ID: 3567360 |
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| Yemul S, Berger C, Estabrook A, Suarez S, Edelson R, Bayley H, Selective killing of T lymphocytes by phototoxic liposomes. Proc Natl Acad Sci U S A84:246-50 1987 |
| PubMed ID: 3491992 |
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| Verhoef V, Fridland A, Metabolic basis of arabinonucleoside selectivity for human leukemic T- and B-lymphoblasts. Cancer Res45:3646-50 1985 |
| PubMed ID: 2410098 |
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| Wellner VP, Anderson ME, Puri RN, Jensen GL, Meister A, Radioprotection by glutathione ester: transport of glutathione ester into human lymphoid cells and fibroblasts. Proc Natl Acad Sci U S A81:4732-5 1984 |
| PubMed ID: 6146978 |
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| Dalla-Favera R, Franchini G, Martinotti S, Wong-Staal F, Gallo RC, Croce CM, Chromosomal assignment of the human homologues of feline sarcoma virus and avian myeloblastosis virus onc genes. Proc Natl Acad Sci U S A79:4714-7 1982 |
| PubMed ID: 6289315 |
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| Lane MA, Sainten A, Cooper GM, Stage-specific transforming genes of human and mouse B- and T- lymphocyte neoplasms. Cell28:873-80 1982 |
| PubMed ID: 6284377 |
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| Daddona PE, Human adenosine deaminase. Properties and turnover in cultured T and B lymphoblasts. J Biol Chem256:12496-501 1981 |
| PubMed ID: 6975278 |
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| Imai K, Galloway DR, Ferrone S, Serological and immunochemical analysis of the specificity of xenoantiserum 8986 elicited with hybrids between human melanoma cells and murine fibroblasts. Cancer Res41:1028-33 1981 |
| PubMed ID: 6161692 |
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| Carrel S, Gross N, Salvi S, Rabbit antiserum against Daudi-cell membrane fractions detects cell surface antigens present on subpopulations of human lymphocytes. J Immunol120:1587-93 1978 |
| PubMed ID: 307019 |
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| Royston I, Graze PR, Pitts RB, Failure of cultured human T-cell lymphoid lines to stimulate in mixed leukocyte culture. J Natl Cancer Inst53:361-7 1974 |
| PubMed ID: 4276467 |
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| Takada A, Takada Y, Minowada J, Immunological functions of human T-lymphoid cell line (MOLT). I. Release of immunosuppressive factors from the mixture of MOLT-4 cells and sheep red blood cells. J Exp Med140:538-48 1974 |
| PubMed ID: 4603015 |
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| Minowada J, Onuma T, Moore GE, Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Natl Cancer Inst49:891-5 1972 |
| PubMed ID: 4567231 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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