GM00467
LCL from B-Lymphocyte
Description:
LESCH-NYHAN SYNDROME; LNS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Disorders of Nucleotide and Nucleic Acid Metabolism |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
HPRT mutation chemically induced in vitro with EMS; selectable with 8-azaguanine or 6-thioguanine |
| Chiorazzi N, Reeves WH, Description and partial characterization of a nucleolar RNA-associated autoantigen defined by a human monoclonal antibody. J Exp Med165:1172-87 1987 |
| PubMed ID: 2435834 |
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| Rosen A, Klein G, UV light-induced immunoglobulin heavy-chain class switch in a human lymphoblastoid cell line. Nature306:189-90 1983 |
| PubMed ID: 6417540 |
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| Chiorazzi N, Wasserman RL, Kunkel HG, Use of Epstein-Barr virus-transformed B cell lines for the generation of immunoglobulin-producing human B cell hybridomas. J Exp Med156:930-5 1982 |
| PubMed ID: 6286839 |
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| Sato K, Slesinski RS, Littlefield JW, Chemical mutagenesis at the phosphoribosyltransferase locus in cultured human lymphoblasts. Proc Natl Acad Sci U S A69:1244-8 1972 |
| PubMed ID: 4504337 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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