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GM00459 Fibroblast from Skin, Unspecified

Description:

CRICETULUS

Affected:

No Data

Sex:

No Data

Age:

No Data

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • External Links
  • Culture Protocols

Overview

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Repository NIGMS Human Genetic Cell Repository
Subcollection Animals
Biopsy Source Unspecified
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Unspecified
Family Member 1
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Cricetulus griseus
Common Name chinese hamster
Remarks Line CHW-1102, MMS induced mutant of GM00458, selected with 8-azaguanine, HPRT deficient; fibroblast culture

Characterizations

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IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 

Phenotypic Data

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Remarks Line CHW-1102, MMS induced mutant of GM00458, selected with 8-azaguanine, HPRT deficient; fibroblast culture

Publications

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Vijayakumar S, Garcia D, Hensel CH, Banerjee M, Bracht T, Xiang R, Kagan J, Naylor SL, The human Y chromosome suppresses the tumorigenicity of PC-3, a human prostate cancer cell line, in athymic nude mice Genes, chromosomes & cancer44:365-72 2005
PubMed ID: 16080199
 
Ono K, Kaku T, Makita N, Kitabatake A, Arita M, Selective block of late currents in the DeltaKPQ Na(+) channel mutant by pilsicainide and lidocaine with distinct mechanisms. Mol Pharmacol57(2):392-400 2000
PubMed ID: 10648650
 
O'Keefe DS, Su SL, Bacich DJ, Horiguchi Y, Luo Y, Powell CT, Zandvliet D, Russell PJ, Molloy PL, Nowak NJ, Shows TB, Mullins C, Vonder Haar RA, Fair WR, Heston WDW, Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene [In Process Citation] Biochim Biophys Acta1443:113-27 1998
PubMed ID: 9838072
 
Raeymaekers P, Van Zand K, Jun L, Hoglund M, Cassiman JJ, Van den Berghe H, Marynen P, A radiation hybrid map with 60 loci covering the entire short arm of chromosome 12. Genomics29:170-8 1995
PubMed ID: 8530068
 
Leach RJ, Chinn R, Reus BE, Hayes S, Schantz L, Dubois B, Overhauser J, Ballabio A, Drabkin H, Lewis TB, et al, Regional localization of 188 sequence tagged sites on a somatic cell hybrid mapping panel for human chromosome 3. Genomics24:549-56 1994
PubMed ID: 7713507
 
O'Connell P, Albertsen H, Matsunami N, Taylor T, Hundley JE, Johnson-Pais TL, Reus B, Lawrence E, Ballard L, White R, et al, A radiation hybrid map of the BRCA1 region. Am J Hum Genet54:526-34 1994
PubMed ID: 8116622
 
Umbricht CB, Griffin CA, Hawkins AL, Grzeschik KH, O'Connell P, Leach R, Green ED, Kelly TJ, High-resolution genomic mapping of the three human replication protein A genes (RPA1, RPA2, and RPA3). Genomics20:249-57 1994
PubMed ID: 8020972
 
Abel KJ, Boehnke M, Prahalad M, Ho P, Flejter WL, Watkins M, VanderStoep J, Chandrasekharappa SC, Collins FS, Glover TW, et al, A radiation hybrid map of the BRCA1 region of chromosome 17q12-q21. Genomics17:632-41 1993
PubMed ID: 8244380
 
Goold RD, diSibio GL, Xu H, Lang DB, Dadgar J, Magrane GG, Dugaiczyk A, Smith KA, Cox DR, Masters SB, et al, The development of sequence-tagged sites for human chromosome 4 [see comments] Hum Mol Genet2:1271-88 1993
PubMed ID: 8401509
 
Washington SS, Bowcock AM, Gerken S, Matsunami N, Lesh D, Osborne-Lawrence SL, Cowell J, Ledbetter DH, White RL, Chakravarti A, A somatic cell hybrid map of human chromosome 13. Genomics18:486-95 1993
PubMed ID: 8307557
 
Craig I, Gemmill R, Kucherlapati R, Report of the first international workshop on human chromosome 12 mapping. Cytogenet Cell Genet61:243-51 1992
PubMed ID: 1362534
 
Kaneko K, Kobayashi H, Onodera O, Miyatake T, Tsuji S, Genomic organization of a cDNA (QM) demonstrating an altered mRNA level in nontumorigenic Wilms' microcell hybrid cells and its localization to Xq28. Hum Mol Genet1:529-33 1992
PubMed ID: 1339145
 
Bols NC, Mosser DD, Boliska SA, Transfer of purine metabolites between cells through the medium and via cell contacts in cocultures of HGPRT+ and HGPRT- cells. Exp Cell Res173:206-17 1987
PubMed ID: 3678380
 
Slater MA, Mosser DD, Bols NC, Established cell lines from different groups of vertebrates undergo metabolic cooperation with one another. In Vitro19:683-92 1983
PubMed ID: 6618511
 
Boliska SA, Bols NC, The transfer of purine metabolites via the medium rather than through cell contacts. Exp Cell Res141:303-12 1982
PubMed ID: 7140841
 
Wang HS, Niewczas V, de S Nazareth HR, Hamerton JL, Cytogenetic characteristics of 26 polyethylene glycol-induced human- hamster hybrid cell lines. Cytogenet Cell Genet24:233-44 1979
PubMed ID: 509993
 
Gee PA, Mohandas RT, Douglas GR, Palser HR, Richardson BJ, Hamerton JL, Characteristics of an HPRT-deficient chinese hamster cell line. Cytogenet Cell Genet13:437-47 1974
PubMed ID: 4462979
 
Douglas GR, McAlpine PJ, Hamerton JL, Regional localization of loci for human PGM and 6PGD on human chromosome one by use of hybrids of Chinese hamster-human somatic cells. Proc Natl Acad Sci U S A70:2737-40 1973
PubMed ID: 4517931

External Links

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dbSNP dbSNP ID: 10338

Culture Protocols

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Split Ratio 1:5
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent
Serum 10% fetal bovine serum Not inactivated
Substrate None specified
Subcultivation Method trypsin-EDTA
Supplement -
Pricing
International/Commercial/For-profit:
$373.00USD
U.S. Academic/Non-profit/Government:
$216.00USD
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