GM00348
Fibroblast from Skin, Unspecified
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Mesocricetus auratus
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Common Name
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golden (or syrian) hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this cell culture gave a negative result with a primer for Yq11, DYS227. |
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| Remarks |
Line B-1; fibroblast culture; thymidine kinase deficient; selectable with 5bromodeoxyuridine; heteroploid |
| Napierala JS, Li Y, Lu Y, Lin K, Hauser LA, Lynch DR, Napierala M, Comprehensive analysis of gene expression patterns in Friedreich's ataxia fibroblasts by RNA sequencing reveals altered levels of protein synthesis factors and solute carriers Disease models & mechanisms10:1353-1369 2017 |
| PubMed ID: 29125828 |
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| McKnight JL, Pellett PE, Jenkins FJ, Roizman B, Characterization and nucleotide sequence of two herpes simplex virus 1 genes whose products modulate alpha-trans-inducing factor-dependent activation of alpha genes. J Virol61:992-1001 1987 |
| PubMed ID: 3029433 |
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| Wilson DE, Woodard D, Sandler A, Erickson J, Gurney A, Provisional assignment of the gene for uridine monophosphatase-2 (Umph- 2) to mouse chromosome 11. Biochem Genet25:1-6 1987 |
| PubMed ID: 3034234 |
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| Pellett PE, McKnight JL, Jenkins FJ, Roizman B, Nucleotide sequence and predicted amino acid sequence of a protein encoded in a small herpes simplex virus DNA fragment capable of trans- inducing alpha genes. Proc Natl Acad Sci U S A82:5870-4 1985 |
| PubMed ID: 2994050 |
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| Kristie TM, Roizman B, Separation of sequences defining basal expression from those conferring alpha gene recognition within the regulatory domains of herpes simplex virus 1 alpha genes. Proc Natl Acad Sci U S A81:4065-9 1984 |
| PubMed ID: 6330737 |
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| Boliska SA, Bols NC, The transfer of purine metabolites via the medium rather than through cell contacts. Exp Cell Res141:303-12 1982 |
| PubMed ID: 7140841 |
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| Tsukamoto K, Klein R, Hatanaka M, Insertion of muntjac gene segment into hamster cells by cell fusion. J Cell Physiol104:225-32 1980 |
| PubMed ID: 7410491 |
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| Littlefield JW, Basilico C, Infection of thymidine kinase-deficient BHK cells with polyoma virus. Nature211:250-2 1966 |
| PubMed ID: 4290606 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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