About this Collection

The Allen Cell Collection is a bank of human induced pluripotent stem cells that are currently derived from the WTC parental line released by the Conklin Laboratory at the J. David Gladstone Institute (available through Coriell as GM25256, where you can also find the de-identified patient information associated with the line). Please refer to the Allen Institute for Cell Science publication for more details on how these cells lines were generated along with the extensive quality control data (Roberts et al., MBoC 2017).

To answer the most basic questions about human health and disease, we need to understand how the dynamic organization and activities of the cell drive its function. This set of powerful gene edited human stem cells from the Allen Institute for Cell Science (AICS) is the first publicly available collection of hiPSC lines with key structural proteins fluorescently tagged. All gene editing and quality control were performed by the Allen Institute for Cell Science.

The Allen Institute’s objective it to provide a new set of lines each quarter. Currently the collection comprises 24 different cell lines targeting 17 key structures. The newest additions to the collection include:

Available in December 2017

     • Autophagosomes: LC3– mEGFP– mono

     • Peroxisomes: PMP34 - mEGFP- mono

     • Endosomes:

          o Rab5 - mEGFP– mono

          o Rab5 - mEGFP– bi

The structures that can now be visualized using lines from the Allen Cell Collection (in alphabetical order) include:

     • Actin filaments: ACTB-mEGFP-mono

     • Actomyosin bundles: MYH10-mEGFP-mono

     • Autophagosomes: LC3-mEGFP– mono

     • Centrosome: CENT2-mTagRFPt - mono

     • Cytoplasm: AAVS1-mEGFP-mono

     • Desmosomes: DSP-mEGFP- mono

     • Endoplasmic reticulum: Sec61-beta-mEGFP-mono

     • Endosomes:

          o Rab5 -mEGFP– mono

          o Rab5– mEGFP– bi

     • Golgi:

          o ST6GAL1-mEGFP-mono

          o ST6GAL1-mEGFP-bi

     • Lysosome: LAMP1-mEGFP-momo

     • Matrix adhesions: PXN-EGFP-mono

     • Microtubules:

          o TUBA1B-mEGFP-mono

          o TUBA1B-mTagRFPt-mono

     • Mitochondria: TOMM20-mEGFP-mono

     • Nuclear Envelope: LMNB1-mEFGP-mono

     • Nucleolus: FBL-mEGFP

     • Peroxisomes: PMP34 - mEGFP- mono

     • Tight junctions: TJP1—mEGFP-mono

     Please pay special attention when ordering either of the Endosome or Golgi lines as the AICS number is now qualified       with a three-digit clonal extension to help discriminate between the mono- and bi-allelic edits of the same structure.

Quality Assurance

Each cell line has undergone a broad range of quality control experiments to ensure precise insertion of the fluorescent tag and localization of the tagged protein to the correct cellular compartment without disrupting the cells’ overall genomic integrity. More details about how these lines are generated and the Quality Control steps involved in their generation are available by clicking on the AICS link provided for each line in the “Order Page” for each cell line.