NG07375
DNA from Fibroblast
Description:
ALZHEIMER DISEASE; AD
Repository
|
NIA Aging Cell Culture Repository
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Subcollection |
Alzheimer's Disease Alzheimer Disease With No Family History |
Quantity |
10 µg |
Quantitation Method |
Please see our FAQ |
Biopsy Source
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Arm
|
Cell Type
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Fibroblast
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Tissue Type
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Skin
|
Transformant
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Untransformed
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Sample Source
|
DNA from Fibroblast
|
Race
|
White
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Freeze |
4.35 |
Passage Frozen |
4 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Remarks |
The donor had a 9 year history of progressive mental deterioration. A CT scan at age 67 showed marked diffuse cortex cerebral atrophy. The skin biopsy was taken ante-mortem from the upper left arm. The culture was initiated on 2/29/84 using explants of minced skin tissue. The cell morphology is fibroblast-like. The karyotype is 46,XY with 6% of the cells examined showing 45,X and 2% showing random chromosome loss. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
Khan TK, Alkon DL, An internally controlled peripheral biomarker for Alzheimer's disease: Erk1 and Erk2 responses to the inflammatory signal bradykinin Proceedings of the National Academy of Sciences of the United States of America103:13203-7 2006 |
PubMed ID: 16920798 |
|
Mazzola JL, Sirover MA, Subcellular alteration of glyceraldehyde-3-phosphate dehydrogenase in Alzheimer's disease fibroblasts Journal of neuroscience research71:279-85 2002 |
PubMed ID: 12503091 |
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Mazzola JL, Sirover MA, Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts. J Neurochem76(2):442-9 2001 |
PubMed ID: 11208907 |
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Hirashima N, Etcheberrigaray R, Bergamaschi S, Racchi M, Battaini F, Binetti G, Govoni S, Alkon DL, Calcium responses in human fibroblasts: a diagnostic molecular profile for Alzheimer's disease [see comments] Neurobiol Aging17:549-55 1996 |
PubMed ID: 8832629 |
|
Kim CS, Han YF, Etcheberrigaray R, Nelson TJ, Olds JL, Yoshioka T, Alkon DL, Alzheimer and beta-amyloid-treated fibroblasts demonstrate a decrease in a memory-associated GTP-binding protein, Cp20. Proc Natl Acad Sci U S A92:3060-4 1995 |
PubMed ID: 7708775 |
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Etcheberrigaray E, Gibson GE, Alkon DL, Molecular mechanisms of memory and the pathophysiology of Alzheimer's disease. Ann N Y Acad Sci747:245-55 1994 |
PubMed ID: 7847674 |
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Ito E, Oka K, Etcheberrigaray R, Nelson TJ, McPhie DL, Tofel-Grehl B, Gibson GE, Alkon DL, Internal Ca2+ mobilization is altered in fibroblasts from patients with Alzheimer disease. Proc Natl Acad Sci U S A91:534-8 1994 |
PubMed ID: 8290560 |
|
Etcheberrigaray R, Ito E, Oka K, Tofel-Grehl B, Gibson GE, Alkon DL, Potassium channel dysfunction in fibroblasts identifies patients with Alzheimer disease. Proc Natl Acad Sci U S A90:8209-13 1993 |
PubMed ID: 8367484 |
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