NG04433
DNA from Fibroblast
Description:
APPARENTLY HEALTHY FETAL TISSUE
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Apparently Healthy Collection |
| Quantity |
10 µg |
| Quantitation Method |
Please see our FAQ |
|
Biopsy Source
|
Buttock-thigh
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
DNA from Fibroblast
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Freeze |
5.08 |
| Passage Frozen |
7 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Remarks |
The culture was initiated on 1/15/80 from explants of minced skin tissue taken from a therapeutically aborted fetus of 19 weeks gestational age. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. A matched lung-derived fibroblast culture from same fetus is AG04434. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Lan YY, Heather JM, Eisenhaure T, Garris CS, Lieb D, Raychowdhury R, Hacohen N, Extranuclear DNA accumulates in aged cells and contributes to senescence and inflammation Aging cell:e12901 2018 |
| PubMed ID: 30706626 |
| |
| Cristofalo VJ, Allen RG, Pignolo RJ, Martin BG, Beck JC, Relationship between donor age and the replicative lifespan of human cells in culture: a reevaluation. Proc Natl Acad Sci U S A95:10614-9 1998 |
| PubMed ID: 9724752 |
| |
| Allen RG, Keogh BP, Gerhard GS, Pignolo R, Horton J, Cristofalo VJ, Expression and regulation of superoxide dismutase activity in human skin fibroblasts from donors of different ages. J Cell Physiol165:576-87 1995 |
| PubMed ID: 7593237 |
| |
| Clemmons DR, Age dependent production of a competence factor by human fibroblasts. J Cell Physiol114:61-7 1983 |
| PubMed ID: 6826661 |
| |
| Jacobs L, Bean CL, Marx JA, Optimal phenotypic expression times for HPRT mutants induced in foreskin-, skin-, and lung-derived human diploid fibroblasts. Environ Mutagen5:717-31 1983 |
| PubMed ID: 6617601 |
|
|