AG27608
iPSC from Fibroblast
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
German Alzheimer Disease |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Tissue Type
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Retroviral)
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Race
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White
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Ethnicity
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German
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Family Member
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17
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Family History
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N
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY[19]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
23 |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remark |
The donor is an unaffected spouse of an Alzheimer's disease family member. Please see Publication Meyer et al.,2019; Parental fibroblast is AG08509. Researchers purchasing hiPSCs from the NIA Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia Japan, Inc. |
| Passage Frozen |
23 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none Not inactivated |
| Substrate |
Matrigel |
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