AG27607
iPSC from Fibroblast
Description:
ALZHEIMER DISEASE; AD
APOLIPOPROTEIN E; APOE
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Tissue Type
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Retroviral)
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Race
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White
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX[24].arr[hg19] 2p22.3p16.3(34,475,819-49,245,264) hmz, 9p21.1q21.13(28,278,250-74,389,006) hmz
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
23 |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remark |
Clinically affected; hiPSC line was derived from externally submitted iPSC line, in which a distribution stock was made. Please see Publication Meyer et al.,2019; Parental fibroblast is AG21158. Researchers purchasing hiPSCs from the NIA Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia Japan, Inc. |
| Meyer K, Feldman HM, Lu T, Drake D, Lim ET, Ling KH, Bishop NA, Pan Y, Seo J, Lin YT, Su SC, Church GM, Tsai LH, Yankner BA, REST and Neural Gene Network Dysregulation in iPSC Models of Alzheimer's Disease Cell reports26:1112-1127.e9 2018 |
| PubMed ID: 30699343 |
| Passage Frozen |
23 |
| Split Ratio |
1:15 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
none |
| Substrate |
Matrigel |
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