Coriell Institute
Repository NIA Aging Cell Culture Repository
Subcollection Alzheimer's Disease
Protocols Protocol PDF
Biopsy Source Arm
Cell Type Stem cell
Tissue Type Induced pluripotent stem cell
Transformant Reprogrammed (Sendai)
Race Caucasian
Ethnicity GERMAN
Family Member 1
Family History Y
Relation to Proband proband
ISCN 46,XX[25].arr(1-22,X)x2
Species Homo sapiens
Common Name Human
Remarks The donor (Volga German) exhibited progressive dementia. Donor's paternal grandfather, aunt, two cousins and two siblings were affected with Alzheimer disease. The affected sister of this donor is AG09907. A heterozygous substitution (A>T at nucleotide 787) in the AD4 [presenilin 2 (PSEN2)] gene results in an Asn141Ile (N141I) missense mutation.
Passage Frozen 14
 
Induced Pluripotent Stem Cell The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.
 
Remark The donor (Volga German) exhibited progressive dementia. Donor's paternal grandfather, aunt, two cousins and two siblings were affected with Alzheimer disease. The affected sister of this donor is AG09907. A heterozygous substitution (A>T at nucleotide 787) in the AD4 [presenilin 2 (PSEN2)] gene results in an Asn141Ile (N141I) missense mutation.
No data is available
No data is available
Passage Frozen 14
Temperature 37 C
Percent CO2 5%
Percent O2 AMBIENT
Medium Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent
Serum 20% Knock-out Serum Replacement
Substrate Gelatin + Feeder Layer

Our mission is to prevent and cure disease through biomedical research.

CONTACT US

CUSTOMER SERVICE
customerservice@coriell.org
(800) 752-3805 • (856) 757-4848

Coriell Institute for Medical Research
403 Haddon Avenue Camden, NJ 08103, USA (856) 966-7377

Ⓒ 2019 Coriell Institute. All rights reserved.