AG20716
Kidney-derived cell line from Kidney
Description:
PEROMYSCUS
ORDER: RODENTIA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Kidney
|
|
Cell Type
|
Kidney-derived cell line
|
|
Tissue Type
|
Kidney
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Kidney-derived cell line from Kidney
|
|
Relation to Proband
|
proband
|
|
Species
|
Peromyscus maniculatus
|
|
Common Name
|
prairie deer mouse
|
|
Remarks
|
|
| Passage Frozen |
11 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Kidney-derived epithelial culture from a 4 month old captive prairie deer mouse (peromyscus maniculatus bairdii) |
| Passage Frozen |
11 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Supplement |
Insulin, Transferrin, Selenium 1% |
|