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AG16086 Fibroblast from Skin, Arm

Description:

GERONTOLOGY RESEARCH CENTER (GRC) CELL CULTURE COLLECTION
APPARENTLY HEALTHY INDIVIDUAL
BALTIMORE LONGITUDINAL STUDY ON AGING (BLSA)

Affected:

No

Sex:

Female

Age:

67 YR (At Sampling)

  • Overview
  • Characterizations
  • Phenotypic Data
  • Publications
  • Culture Protocols

Overview

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Repository NIA Aging Cell Culture Repository
Subcollection GRC
Apparently Healthy Collection
Biopsy Source Arm
Cell Type Fibroblast
Tissue Type Skin
Transformant Untransformed
Sample Source Fibroblast from Skin, Arm
Race Asian
Ethnicity FILIPINO
Relation to Proband proband
Confirmation Clinical summary/Case history
Species Homo sapiens
Common Name Human
Remarks The biopsy site is the mesial aspect of the mid-upper left arm, taken ante-mortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XX with 8% of the cells examined showing random chromosome loss/gain and 4% showing random chromosomal aberrations. Same subject as AG28262 (iPSC). The legacy karyotype description shown in this Remark may not be representative of the current available product.

Characterizations

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PDL at Freeze 7.34
Passage Frozen 8
 
IDENTIFICATION OF SPECIES OF ORIGIN Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis
 

Phenotypic Data

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Remarks The biopsy site is the mesial aspect of the mid-upper left arm, taken ante-mortem. The primary culture was initiated from explants of a 2 mm-punch biopsy. The cell morphology is fibroblast-like and the karyotype is 46,XX with 8% of the cells examined showing random chromosome loss/gain and 4% showing random chromosomal aberrations. Same subject as AG28262 (iPSC). The legacy karyotype description shown in this Remark may not be representative of the current available product.

Publications

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Fafián-Labora JA, O'Loghlen A, NF-?B/IKK activation by small extracellular vesicles within the SASP Aging cell20:e13426 2021
PubMed ID: 34187082
 
Fafián-Labora JA, Rodríguez-Navarro JA, O'Loghlen A, Small Extracellular Vesicles Have GST Activity and Ameliorate Senescence-Related Tissue Damage Cell metabolism20:e13426 2020
PubMed ID: 32574561
 
Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19:e13426 2018
PubMed ID: 30567591

Culture Protocols

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Cumulative PDL at Freeze 13.34
Passage Frozen 8
Split Ratio 1:3
Temperature 37 C
Percent CO2 5%
Percent O2 3%
Medium Eagles Minimum Essential Medium with Earle's salts:Dulbecco's modified MEM with 2mM L-glutamine or equivalent
Serum 15% fetal bovine serum Not inactivated
Substrate Gelatin
Supplement -
Pricing
Commercial:
$257.00USD
Academic &
Non-profit:
$103.00USD
NIA Grantees:
$47.00USD
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Related Products
Same Subject
  • NG16086 - DNA
  • AG28262 - Stem cell
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