AG15865
Fibroblast from Artery, Aorta, thoracic
Description:
RATTUS
ORDER: RODENTIA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Fibroblast
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Artery, Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XY[41]/42,XY,t(7;11)(q11;q23)[2]/43,XY,+12[7]
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Species
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Rattus norvegicus
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Common Name
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brown or Norway rat
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Remarks
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| PDL at Senescence |
203 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Sprague Dawley. Culture initiated on 3/5/99 from fibroblasts grown out from minced aortic fragments. Cell morphology is fibroblast-like. The culture is a mosiac with karyotype: 42,XY[41]/42,XY,t(7;11)(q11;q23)[2]/43,XY,+12[7] with 6% of the cells examined showing random loss/gain. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| PDL at Senescence |
203 |
| Passage Frozen |
4 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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