AG08503
Endothelial from Aorta, thoracic
Description:
BOS
ORDER: CETARTIODACTYLA
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Animals |
Biopsy Source
|
Aorta, thoracic
|
Cell Type
|
Endothelial
|
Tissue Type
|
Artery
|
Transformant
|
Untransformed
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
60,XY
|
Species
|
Bos taurus
|
Common Name
|
cow
|
Remarks
|
|
PDL at Senescence |
70 |
PDL at Freeze |
6 |
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Genbank |
COX1 |
Remark |
Angus breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 10/22/85. The culture was initiated from cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The karyotype is 60,XY; normal diploid male. The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The first and second passage cells were propagated in medium supplemented with horse plasma-derived serum and heparin (0.1mg/ ml) on gelatin-coated flasks. The culture was grown on gelatin-coated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). Culture medium: AMMC 20U with supplements. For lifespan determination this cell line was cultured in Ham's F12 medium with 10% inactivated fetal bovine serum. A smooth muscle cell culture from this calf is AG08504A. This cell line will only be shipped frozen. |
PDL at Senescence |
70 |
Cumulative PDL at Freeze |
6 |
Passage Frozen |
2 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
MEM (Eagle) Alpha Modification with nucleosides/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent |
Serum |
20% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
Supplement |
Heparin 0.05 mg/ml |
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