AG08495
Fibroblast from Skin, Arm
Description:
MACACA NEMESTRINA
ORDER: PRIMATES
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Nonhuman Primates Animals |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Arm
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
42,XY
|
Species
|
Macaca nemestrina
|
Common Name
|
pigtailed macaque
|
Remarks
|
|
PDL at Senescence |
9 |
PDL at Freeze |
3 |
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
|
Genbank |
COX1 |
Remarks |
The skin biopsy was taken ante-mortem on 10/30/85 from the inner aspect of the upper left arm. |
PDL at Senescence |
9 |
Cumulative PDL at Freeze |
3 |
Passage Frozen |
2 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|