AG08318
Smooth muscle from Aorta, thoracic
Description:
MACACA MULATTA
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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42,XX,abnormal 15
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Species
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Macaca mulatta
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Common Name
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rhesus monkey
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Remarks
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| PDL at Senescence |
47 |
| PDL at Freeze |
18 |
| Passage Frozen |
7 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The age of the donor is between 1 and 2 years. The thoracic aorta was removed post-mortem on 7/9/85. A matching lung fibroblast culture from this monkey is AG08316. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). |
| PDL at Senescence |
47 |
| Cumulative PDL at Freeze |
18 |
| Passage Frozen |
7 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 medium/Medium 199, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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